Difference between revisions of "20.109(S08): TA notes for module 1"
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(→General notes) |
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*pCX-EGFP: | *pCX-EGFP: | ||
| − | * | + | ** contains full-length EGFP gene |
| − | * | + | ** Amp resistant |
| − | * | + | ** f1 ori |
| − | * | + | ** SV40 ori |
| − | + | ||
*pCX-NNX: | *pCX-NNX: | ||
| − | * | + | ** a mock version of pCX-EGFP, contains no EGFP gene |
| − | * | + | ** Amp resistant |
| − | * | + | ** f1 ori |
| − | * | + | ** some altered restriction sites compared to pCX-EGFP, and SV40 ori deleted |
==Daily Notes== | ==Daily Notes== | ||
Revision as of 19:29, 27 December 2007
General notes
Plasmids used:
- pCX-EGFP:
- contains full-length EGFP gene
- Amp resistant
- f1 ori
- SV40 ori
- pCX-NNX:
- a mock version of pCX-EGFP, contains no EGFP gene
- Amp resistant
- f1 ori
- some altered restriction sites compared to pCX-EGFP, and SV40 ori deleted
Daily Notes
Day 1
Materials required:
- PCR Master Mix (2.5X), ~ 50 μL per group
- DI water, prep 100 μL aliquot per group
- 5 μL aliquots each of pCX-EGFP, D32N-fwd, D32N-rev
- 2 PCR tubes per group
Keep everything cold!
Day of Lab:
- Lab orientation quiz (not a TA quiz) will be taken today.
- Remember to freeze PCR products when they are ready.
