Difference between revisions of "20.109(S08): TA notes for module 1"
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===[[20.109%28S08%29:DNA_engineering/DNA_engineering_by_PCR | Day 1]]=== | ===[[20.109%28S08%29:DNA_engineering/DNA_engineering_by_PCR | Day 1]]=== | ||
| − | Materials required: | + | '''Materials required:''' |
#PCR Master Mix (2.5X), ~ 50 μL per group | #PCR Master Mix (2.5X), ~ 50 μL per group | ||
#DI water, prep 100 μL aliquot per group | #DI water, prep 100 μL aliquot per group | ||
#5 μL aliquots each of pCX-EGFP, D32N-fwd, D32N-rev | #5 μL aliquots each of pCX-EGFP, D32N-fwd, D32N-rev | ||
| − | # 2 PCR tubes per group | + | #2 PCR tubes per group |
Keep everything cold! | Keep everything cold! | ||
| − | Day of Lab: | + | '''Day of Lab:''' |
#Lab orientation quiz (not a TA quiz) will be taken today. | #Lab orientation quiz (not a TA quiz) will be taken today. | ||
#Remember to freeze PCR products when they are ready. | #Remember to freeze PCR products when they are ready. | ||
Revision as of 19:12, 27 December 2007
General notes
Daily Notes
Day 1
Materials required:
- PCR Master Mix (2.5X), ~ 50 μL per group
- DI water, prep 100 μL aliquot per group
- 5 μL aliquots each of pCX-EGFP, D32N-fwd, D32N-rev
- 2 PCR tubes per group
Keep everything cold!
Day of Lab:
- Lab orientation quiz (not a TA quiz) will be taken today.
- Remember to freeze PCR products when they are ready.
