Difference between revisions of "20.109(S07): Bio-material engineering/Optimizing panning"
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==Introduction== | ==Introduction== | ||
− | Thanks to recent advances in DNA synthesis chemistry, high quality pools of oligonucleotides are available at reasonable concentrations and prices. A quick look around the Internet for companies performing DNA synthesis (companies such as Integrated DNA Technologies or Operon) shows that | + | Thanks to recent advances in DNA synthesis chemistry, high quality pools of oligonucleotides are available at reasonable concentrations and prices. A quick look around the Internet for companies performing DNA synthesis (companies such as Integrated DNA Technologies or Operon) shows that it s possible to get next day delivery on a 20 or 30 base sequence of your choosing for around 20 dollars. The synthesis chemistry requires only a few things: a solid support on which to build the oligos, nucleotides that can be protected from further nucleotide additions, a way of deprotecting the nucleotides once they ve been added to the oligo chain, and finally a way of releasing the finished oligos from the support at the end of the synthesis. An automated synthesizer and a schematic of synthesis chemistry are shown. |
[[Image:Be109synthesizer.jpg |thumb|left|250px| '''DNA synthesizer''']] | [[Image:Be109synthesizer.jpg |thumb|left|250px| '''DNA synthesizer''']] | ||
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− | Consider making a 4-mer: GATC. To begin the synthesis, the | + | Consider making a 4-mer: GATC. To begin the synthesis, the 3 hydroxyl group of the first nucleotide (guanosine) would be coupled to a 500 angstrom bead ( |