OD562 absorbance measurements for protein concentration calculations
During the 1 hour incubation of Part 4A use Microsoft Excel to prepare a graph of absorbance versus BSA concentration using the sample data here. You will need to use the trendline equation to calculate the protein concentration of your example elution data. You may also find this technical bulletin helpful.
T/R Class Data
TR Class microBCA assay data here.
W/F red team
| name
|
OD562
|
| standards
|
| A
|
3.02
|
| B
|
0.798
|
| C
|
0.444
|
| D
|
0.245
|
| E
|
0.141
|
| F
|
0.073
|
| G
|
0.054
|
| H
|
0.034
|
| inverse pericam (IPC)
|
| wild-type
|
0.177
|
| mutant
|
0.201
|
W/F orange team
| name
|
OD562
|
| standards
|
| A
|
2.970
|
| B
|
0.672
|
| C
|
0.337
|
| D
|
0.174
|
| E
|
0.086
|
| F
|
0.037
|
| G
|
0.018
|
| H
|
0.000
|
| inverse pericam (IPC)
|
| wild-type
|
0.028
|
| mutant
|
0.062
|
W/F blue team
| name
|
OD562
|
| standards
|
| A
|
2.983
|
| B
|
0.727
|
| C
|
0.292
|
| D
|
0.168
|
| E
|
0.091
|
| F
|
0.034
|
| G
|
0.015
|
| H
|
0.000
|
| inverse pericam (IPC)
|
| wild-type
|
0.049
|
| mutant
|
0.027
|
W/F pink team
| name
|
OD562
|
| standards
|
| A
|
2.943
|
| B
|
0.699
|
| C
|
0.341
|
| D
|
0.166
|
| E
|
0.101
|
| F
|
0.038
|
| G
|
0.017
|
| H
|
0.000
|
| inverse pericam (IPC)
|
| wild-type
|
0.025
|
| mutant
|
0.136
|
W/F purple team
| name
|
OD562
|
| standards
|
| A
|
2.948
|
| B
|
0.812
|
| C
|
0.420
|
| D
|
0.214
|
| E
|
0.118
|
| F
|
0.047
|
| G
|
0.026
|
| H
|
0.000
|
| inverse pericam (IPC)
|
| wild-type
|
0.016
|
| mutant
|
0.010
|
