Talk:20.109(F16):Design gRNA for CRISPRi (Day2)
From Course Wiki
Fermentation product and gene targeted:
T/R
| Team | Ethanol (E) or lactate (L) | Gene targeted by CRISPRi gRNA | gRNA sequence (without tag at 3' end) | ||||
| yellow | L | pflB | TGTCGAAGTACGCAGTAAAT | ||||
| green | L | pflB | ATAAAAAATCCACTTAAGAAGGTA | ||||
| blue | E | pta | ttcacgacaacgttcaataatcat | ||||
| pink | L | pflB | AAATAAAAAATCCACTTAAGAAGGT | ||||
| purple | L | pflB | AAATCCACTTAAGAAGGTAGGTGT | white | L | pflB | AAATAAAAAATCCACTTAAGAAGGT |
W/F
| Team | Ethanol (E) or lactate (L) | Gene targeted by CRISPRi gRNA | gRNA sequence (without tag at 3' end) |
| red | E | pta-ack | GCC ACG TAT CAA TTA TAG GTA C |
| green | E | ldhA | GTAGCTTAAATGTGATTCAACATC |
| blue | E | fdrA | CAAGATCGGCTTGAAAGGTTTGCAC |
| purple | E | ldhA | TCGTACTGTTTTGTGCTATAAA |
