Spring 11:Directed Evolution/KAF95

From Course Wiki
Jump to: navigation, search

Notes

Genotype: Δ(cheY)m43-10, fliC726

Exhibits type1 pili. Possible fliC is on a plasmid

hag (standing for H, antigen) was the former name for fliC.


References

  • "(I worked most extensively with pFD313, a pBR322-based plasmid which contains a deletion of 171 nucleotides in the central region of flicC, encoding a 46,427 Da product. Wild-type flagellin is a 497-residue protein of 51,172 Da (Kuwajima et al., 1986)." Strain is by Karen Alicia Fahrner (Studies of Bacterial Flagellar Motors and Filaments) (1995).
  • "Strain KAF95 carries a cheY deletion (m43-10, from strain RP4979, the gift of Sandy Parkinson) and, thus, swims smoothly (rotates its flagella exclusively CCW)."Berg, Turner 1993
Retrieved from "http://engineerbiology.org/w/index.php?title=Spring_11:Directed_Evolution/KAF95&oldid=3501"