20.109(F07):Module 1:RefactorM13

20.109(F07): Laboratory Fundamentals of Biological Engineering

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Genome Engineering Expression Engineering Biomaterials Engineering

All parts are specified at the Registry of Standard Biological Parts. Refine the parts there as well as here, adding your comments to the area below the table.

genetic element	promoter	RBS	coding
	start synthesis with
gII	BBa_M13102 (need 5' UTR?)	BBa_M13502	BBa_M13002' (modified to remove gene 10 promoter)
gX	BBa_M13110 (need 5' UTR?)	BBa_M13510	BBa_M13010' (modified to remove gene 5 promoter)
gV	BBa_M13105 (need 5' UTR?)	BBa_M13505	BBa_M13005
gVII		BBa_M13507	BBa_M13007' (modified to remove overlap with gene 9 dwnstm)
gIX		BBa_M13509	BBa_M13009' (modified to remove overlap with gene 8 dwnstm)
gVIII	BBa_M13108 (need 5' UTR?)	BBa_M13508	BBa_M13008
	Transcriptional terminator (if M13K07 part, then need to modify to remove gene 3 promoter)
gIII	BBa_M13103	BBa_M13503	BBa_M13003' (modified to remove gene 6 promoter, change GTG start?)
gVI	BBa_M13106	BBa_M13506	BBa_M13006' (modified to remove gene 1 promoter)
gI	BBa_M13101	BBa_M13501	BBa_M13001' (modified to remove gene 11 RBS, gene 4 promoter, RBS, start)
gXI		BBa_M13511	BBa_M13011' (modified to remove gene 4 promoter, RBS, start)
gIV	BBa_M13104 (need 5' UTR?)	BBa_M13504	BBa_M13004'
	M13K07 ori/KanR/p15a ori
	end synthesis

Note: modified parts codon varied to remove direct repeats.

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