Difference between revisions of "User:Kevinly"
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== Assignment 1: Optics boot camp == | == Assignment 1: Optics boot camp == | ||
| − | + | ; Overview | |
| − | + | # Work on mystery negative lens on paper before going to the lens station | |
| + | # Verify lens-maker formula | ||
| + | # Become familiar with MATLAB indexing and plotting of single/multiple pixels | ||
| + | |||
| + | ; Lab notes | ||
| + | * The lens-maker-verifying component is already built and tucked away in the cubbies in the lab | ||
== Assignment 2: Brightfield transillumination microscope == | == Assignment 2: Brightfield transillumination microscope == | ||
| + | ; Overview | ||
| + | |||
| + | ; Coding notes | ||
| + | |||
| + | ; Lab notes | ||
| + | * Aligning the aluminum mirror | ||
== Assignment 3: Single-color epifluorescent microscope == | == Assignment 3: Single-color epifluorescent microscope == | ||
== Assignment 4: Resolution and particle tracking in glycerin == | == Assignment 4: Resolution and particle tracking in glycerin == | ||
| − | - | + | ; Milestones |
| + | * Measure the resolution of their optical system (40x Nikon with 125mm tube lens and 590nm light); '''Expect resolution around 625nm''' | ||
| + | |||
| + | ; Lab notes | ||
| + | * The PSF beads for measuring the resolution are particularly small | ||
| + | ** Set the gain to max and use a exposure of about 200,000 µs when focusing and searching for a good patch of beads | ||
| + | ** When you actually take the PSF image, you can lower the gain and expect an exposure of about 800,000 µs (with current around 0.5 A) | ||
| + | ** Some slides are better prepared than others (the older slides are photobleached) | ||
| + | |||
| + | * Preparing the sample slide with particles diffusing in glycerin | ||
| + | ** Make a channel on a glass slide using two double-sided tape with a cover slip sandwiched on top. Then let the glycerin-bead mixture capillary action its way through before sealing each end of the channel with nail polish | ||
| + | ** You'll have to let the slide sit for a while because there may still be flow from the capillary action movement | ||
| + | *** Even if there is flow, you can account for it using the difference trajectory | ||
| + | ** Its better to do this with higher concentration of beads (10~30 in the FOV) | ||
== Assignment 5: Particle tracking in cells == | == Assignment 5: Particle tracking in cells == | ||
- | - | ||
This Assignment is known for applying the particle tracking code from Assignment 4. | This Assignment is known for applying the particle tracking code from Assignment 4. | ||
| + | |||
| + | == Lab Chore Tutorials == | ||
| + | ; Cleaning camera sensors | ||
| + | With the new camera sensor wipers that Steve ordered (they're actually designed for DSLR camera sensors), this Lab Chore is much easier (and almost fun). The traditional method was to use [lens cleaning tissue](https://www.thorlabs.com/newgrouppage9.cfm?objectgroup_ID=330), but sometimes that left lint or deposited even more dirt. '''The key with cleaning lenses and sensors is to firmly wipe it once'''. | ||
| + | |||
| + | * New cleaning method: | ||
| + | *# Wet one side of the wiper with the cleaner fluid | ||
| + | * Old cleaning method: | ||
| + | * General cleaning tips: | ||
| + | ** Never have the exposed camera sensor angled upward; dirt and dust settles downwards! | ||
| + | ** Wipe once | ||
| + | ** | ||
| + | |||
| + | ; Set screw guidelines | ||
| + | There are a handful of microscope parts that use set screws: C6W, CP02, and the cage rods. | ||
Revision as of 04:32, 14 October 2022
This Wiki page is a comprehensive compilation of Kevin's personal memos for each Assignment and component of 20.309. The intended audience is just Kevin, but if others read it and happen to learn from it, then that's nice too. Perhaps in the future, when there is a new generation of 20.309 instructors, these logs and memos will be a valuable resource.
This page is loosely organized by Assignments/Labs.
Contents
Assignment 1: Optics boot camp
- Overview
- Work on mystery negative lens on paper before going to the lens station
- Verify lens-maker formula
- Become familiar with MATLAB indexing and plotting of single/multiple pixels
- Lab notes
- The lens-maker-verifying component is already built and tucked away in the cubbies in the lab
Assignment 2: Brightfield transillumination microscope
- Overview
- Coding notes
- Lab notes
- Aligning the aluminum mirror
Assignment 3: Single-color epifluorescent microscope
Assignment 4: Resolution and particle tracking in glycerin
- Milestones
- Measure the resolution of their optical system (40x Nikon with 125mm tube lens and 590nm light); Expect resolution around 625nm
- Lab notes
- The PSF beads for measuring the resolution are particularly small
- Set the gain to max and use a exposure of about 200,000 µs when focusing and searching for a good patch of beads
- When you actually take the PSF image, you can lower the gain and expect an exposure of about 800,000 µs (with current around 0.5 A)
- Some slides are better prepared than others (the older slides are photobleached)
- Preparing the sample slide with particles diffusing in glycerin
- Make a channel on a glass slide using two double-sided tape with a cover slip sandwiched on top. Then let the glycerin-bead mixture capillary action its way through before sealing each end of the channel with nail polish
- You'll have to let the slide sit for a while because there may still be flow from the capillary action movement
- Even if there is flow, you can account for it using the difference trajectory
- Its better to do this with higher concentration of beads (10~30 in the FOV)
Assignment 5: Particle tracking in cells
- This Assignment is known for applying the particle tracking code from Assignment 4.
Lab Chore Tutorials
- Cleaning camera sensors
With the new camera sensor wipers that Steve ordered (they're actually designed for DSLR camera sensors), this Lab Chore is much easier (and almost fun). The traditional method was to use [lens cleaning tissue](https://www.thorlabs.com/newgrouppage9.cfm?objectgroup_ID=330), but sometimes that left lint or deposited even more dirt. The key with cleaning lenses and sensors is to firmly wipe it once.
- New cleaning method:
- Wet one side of the wiper with the cleaner fluid
- Old cleaning method:
- General cleaning tips:
- Never have the exposed camera sensor angled upward; dirt and dust settles downwards!
- Wipe once
- Set screw guidelines
There are a handful of microscope parts that use set screws: C6W, CP02, and the cage rods.
