Difference between revisions of "Thermal Quenching Model"
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(Created page with "Fee fi fo fum! Zipper et al says linear. Where the hell was that other paper that said linear") |
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Zipper et al says linear. | Zipper et al says linear. | ||
| − | + | The Roche LightCycler paper [https://www.roche-applied-science.com/PROD_INF/BIOCHEMI/No.2_98/p8-11.pdf] also shows a linear dependence, or at least it looks pretty linear (Fig. 2). Interesting -- they have a melting temp of about 85 degrees, with just normal DNA of 536bp length. We should try that. Anyway, my suspicion is that "linear" is a fine model of thermal quenching, and we should de-prioritize thermal quenching investigations. | |
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| + | '''Polymers & stuff''' | ||
| + | |||
| + | * Polyvinyl alcohol | ||
| + | * Polyvinylpyrrolidone | ||
| + | * Polyethylene glycol (?) | ||
| + | * Polysorbate (?) | ||
| + | * Polyvinyl acetate (?) | ||
| + | * Matrigel | ||
| + | * Mineral oil | ||
| + | * Glycerol | ||
| + | * Gelatin | ||
| + | * Agar(ose) | ||
Latest revision as of 22:23, 22 July 2011
Zipper et al says linear.
The Roche LightCycler paper [1] also shows a linear dependence, or at least it looks pretty linear (Fig. 2). Interesting -- they have a melting temp of about 85 degrees, with just normal DNA of 536bp length. We should try that. Anyway, my suspicion is that "linear" is a fine model of thermal quenching, and we should de-prioritize thermal quenching investigations.
Polymers & stuff
- Polyvinyl alcohol
- Polyvinylpyrrolidone
- Polyethylene glycol (?)
- Polysorbate (?)
- Polyvinyl acetate (?)
- Matrigel
- Mineral oil
- Glycerol
- Gelatin
- Agar(ose)
