Difference between revisions of "Talk:20.109(S16):Purify protein (Day6)"

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(→‎T/R red team)
Line 40: Line 40:
 
|0.201
 
|0.201
 
|}
 
|}
 +
  
 
====<font color="red">W/F red team</font color>====
 
====<font color="red">W/F red team</font color>====
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|0.201
 
|0.201
 
|}
 
|}
−
 
  
 
====<font color=#FF6600>W/F orange team</font color>====
 
====<font color=#FF6600>W/F orange team</font color>====
 +
{| border="1"
 +
|'''name'''
 +
|'''OD562'''
 +
|-
 +
|colspan=2|standards
 +
|-
 +
|A
 +
|3.02
 +
|-
 +
|B
 +
|0.798
 +
|-
 +
|C
 +
|0.444
 +
|-
 +
|D
 +
|0.245
 +
|-
 +
|E
 +
|0.141
 +
|-
 +
|F
 +
|0.073
 +
|-
 +
|G
 +
|0.054
 +
|-
 +
|H
 +
|0.034
 +
|-
 +
|colspan=2| inverse pericam (IPC)
 +
|-
 +
|wild-type
 +
|0.177
 +
|-
 +
|mutant
 +
|0.201
 +
|}
 +
 +
====<font color="blue">W/F blue team</font color>====
 +
{| border="1"
 +
|'''name'''
 +
|'''OD562'''
 +
|-
 +
|colspan=2|standards
 +
|-
 +
|A
 +
|3.02
 +
|-
 +
|B
 +
|0.798
 +
|-
 +
|C
 +
|0.444
 +
|-
 +
|D
 +
|0.245
 +
|-
 +
|E
 +
|0.141
 +
|-
 +
|F
 +
|0.073
 +
|-
 +
|G
 +
|0.054
 +
|-
 +
|H
 +
|0.034
 +
|-
 +
|colspan=2| inverse pericam (IPC)
 +
|-
 +
|wild-type
 +
|0.177
 +
|-
 +
|mutant
 +
|0.201
 +
|}
 +
 +
====<font color=#FF66CC>W/F pink team</font color>====
 +
{| border="1"
 +
|'''name'''
 +
|'''OD562'''
 +
|-
 +
|colspan=2|standards
 +
|-
 +
|A
 +
|3.02
 +
|-
 +
|B
 +
|0.798
 +
|-
 +
|C
 +
|0.444
 +
|-
 +
|D
 +
|0.245
 +
|-
 +
|E
 +
|0.141
 +
|-
 +
|F
 +
|0.073
 +
|-
 +
|G
 +
|0.054
 +
|-
 +
|H
 +
|0.034
 +
|-
 +
|colspan=2| inverse pericam (IPC)
 +
|-
 +
|wild-type
 +
|0.177
 +
|-
 +
|mutant
 +
|0.201
 +
|}
 +
 +
====<font color=#9933FF>W/F purple team</font color>====
 
{| border="1"
 
{| border="1"
 
|'''name'''
 
|'''name'''

Revision as of 21:55, 26 February 2016

OD562 absorbance measurements for protein concentration calculations

During the 1 hour incubation of Part 4A use Microsoft Excel to prepare a graph of absorbance versus BSA concentration using the sample data here. You will need to use the trendline equation to calculate the protein concentration of your example elution data. You may also find this technical bulletin helpful.

T/R red team

name OD562
standards
A 3.02
B 0.798
C 0.444
D 0.245
E 0.141
F 0.073
G 0.054
H 0.034
inverse pericam (IPC)
wild-type 0.177
mutant 0.201


W/F red team

name OD562
standards
A 3.02
B 0.798
C 0.444
D 0.245
E 0.141
F 0.073
G 0.054
H 0.034
inverse pericam (IPC)
wild-type 0.177
mutant 0.201

W/F orange team

name OD562
standards
A 3.02
B 0.798
C 0.444
D 0.245
E 0.141
F 0.073
G 0.054
H 0.034
inverse pericam (IPC)
wild-type 0.177
mutant 0.201

W/F blue team

name OD562
standards
A 3.02
B 0.798
C 0.444
D 0.245
E 0.141
F 0.073
G 0.054
H 0.034
inverse pericam (IPC)
wild-type 0.177
mutant 0.201

W/F pink team

name OD562
standards
A 3.02
B 0.798
C 0.444
D 0.245
E 0.141
F 0.073
G 0.054
H 0.034
inverse pericam (IPC)
wild-type 0.177
mutant 0.201

W/F purple team

name OD562
standards
A 3.02
B 0.798
C 0.444
D 0.245
E 0.141
F 0.073
G 0.054
H 0.034
inverse pericam (IPC)
wild-type 0.177
mutant 0.201
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