Difference between revisions of "Talk:20.109(S16):Purify protein (Day6)"

From Course Wiki
Jump to: navigation, search
m (→‎OD562 absorbance measurements for protein concentration calculations)
(→‎T/R red team)
Line 30: Line 30:
 
|-
 
|-
 
|I
 
|I
 +
|0.034
 +
|-
 +
|colspan=2| inverse pericam (IPC)
 +
|-
 +
|wild-type
 +
|0.177
 +
|-
 +
|mutant
 +
|0.201
 +
|}
 +
 +
====<font color="red">W/F red team</font color>====
 +
{| border="1"
 +
|'''name'''
 +
|'''OD562'''
 +
|-
 +
|colspan=2|standards
 +
|-
 +
|A
 +
|3.02
 +
|-
 +
|B
 +
|0.798
 +
|-
 +
|C
 +
|0.444
 +
|-
 +
|D
 +
|0.245
 +
|-
 +
|E
 +
|0.141
 +
|-
 +
|F
 +
|0.073
 +
|-
 +
|G
 +
|0.054
 +
|-
 +
|H
 +
|0.034
 +
|-
 +
|colspan=2| inverse pericam (IPC)
 +
|-
 +
|wild-type
 +
|0.177
 +
|-
 +
|mutant
 +
|0.201
 +
|}
 +
 +
 +
====<font color=#FF6600>W/F orange team</font color>====
 +
{| border="1"
 +
|'''name'''
 +
|'''OD562'''
 +
|-
 +
|colspan=2|standards
 +
|-
 +
|A
 +
|3.02
 +
|-
 +
|B
 +
|0.798
 +
|-
 +
|C
 +
|0.444
 +
|-
 +
|D
 +
|0.245
 +
|-
 +
|E
 +
|0.141
 +
|-
 +
|F
 +
|0.073
 +
|-
 +
|G
 +
|0.054
 +
|-
 +
|H
 
|0.034
 
|0.034
 
|-
 
|-

Revision as of 21:44, 26 February 2016

OD562 absorbance measurements for protein concentration calculations

During the 1 hour incubation of Part 4A use Microsoft Excel to prepare a graph of absorbance versus BSA concentration using the sample data here. You will need to use the trendline equation to calculate the protein concentration of your example elution data. You may also find this technical bulletin helpful.

T/R red team

name OD562
standards
A 3.02
B 0.798
C 0.444
D 0.245
E 0.141
F 0.073
G 0.054
I 0.034
inverse pericam (IPC)
wild-type 0.177
mutant 0.201

W/F red team

name OD562
standards
A 3.02
B 0.798
C 0.444
D 0.245
E 0.141
F 0.073
G 0.054
H 0.034
inverse pericam (IPC)
wild-type 0.177
mutant 0.201


W/F orange team

name OD562
standards
A 3.02
B 0.798
C 0.444
D 0.245
E 0.141
F 0.073
G 0.054
H 0.034
inverse pericam (IPC)
wild-type 0.177
mutant 0.201
Retrieved from "http://engineerbiology.org/w/index.php?title=Talk:20.109(S16):Purify_protein_(Day6)&oldid=30177"