Difference between revisions of "Talk:20.109(F17):Module 2"
From Course Wiki
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|GCTGTGGGATAAAAACAATCTGGAG | |GCTGTGGGATAAAAACAATCTGGAG | ||
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| − | | | + | |template strand |
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|green | |green | ||
Revision as of 19:08, 2 November 2017
Contents
Fermentation product and gene targeted:
T/R
| Team | Ethanol (E) or Acetate (A) | Gene targeted by CRISPRi gRNA | gRNA sequence (without tag at 3' end) | Locus targeted (eg. beginning of gene, putative promoter, -35 region) | Target template or nontemplate strand |
| red | Acetate | adhE | TTAACGCACTCGTAGAGCGTGTAAA | ||
| orange | Ethanol | pta-ack | CTATGGCTCCCTGACGTTTT | ||
| yellow | Ethanol | frdA | GCTGTGGGATAAAAACAATCTGGAG | minus 35 region | template strand |
| green | E | LdhA | ttgtgctataaacggcgagtttcat | ||
| blue | E | pta | ttcacgacaacgttcaataatcat | ||
| pink | Acetate | adhE | TTACTAAAAAAGTTTAACATTATCA | promoter region | template strand |
| purple | Acetate | adhE | TTTACTAAAAAAGTTTAACATTATC | ||
| white | Acetate | adhE | CTGATAATGTTAAACTTTTT |
W/F
| Team | Ethanol (E) or Acetate (A) | Gene targeted by CRISPRi gRNA | gRNA sequence (without tag at 3' end) | Locus targeted (eg. beginning of gene, putative promoter, -35 region) | Target template or nontemplate strand |
| red | Ethanol | ack (indirectly, pta) | GTTTTTTTAGCCACGTATCAATTAT | ||
| orange | Ethanol | ldhA | ATTCAACATCACTGGAGAAAGTCTT | ||
| blue | Ethanol | ackA | TTTTTAGCCACGTATCAATTAT |
M2D5/M2D7: sequencing data
T/R
| Team | pgRNA sequencing |
| red | files |
| orange | files |
| yellow | files |
| green | files |
| blue | files |
| pink | files |
| purple | files |
| white | files |
W/F
| Team | pgRNA sequencing |
| red | files |
| orange | files |
| blue | files |
