Difference between revisions of "Talk:20.109(F17):Module 2"
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(→Fermentation product and gene targeted:) |
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| Line 8: | Line 8: | ||
|'''Gene targeted by CRISPRi gRNA''' | |'''Gene targeted by CRISPRi gRNA''' | ||
|'''gRNA sequence (without tag at 3' end)''' | |'''gRNA sequence (without tag at 3' end)''' | ||
| + | |'''Locus targeted (eg. beginning of gene, putative promoter, -35 region)''' | ||
| + | |'''Target template or nontemplate strand''' | ||
|- | |- | ||
|red | |red | ||
| Line 13: | Line 15: | ||
| adhE | | adhE | ||
| TTAACGCACTCGTAGAGCGTGTAAA | | TTAACGCACTCGTAGAGCGTGTAAA | ||
| + | | | ||
| + | | | ||
|- | |- | ||
|orange | |orange | ||
| Line 18: | Line 22: | ||
|pta-ack | |pta-ack | ||
|CTATGGCTCCCTGACGTTTT | |CTATGGCTCCCTGACGTTTT | ||
| + | | | ||
| + | | | ||
|- | |- | ||
|yellow | |yellow | ||
| Line 23: | Line 29: | ||
|frdA | |frdA | ||
|GCTGTGGGATAAAAACAATCTGGAG | |GCTGTGGGATAAAAACAATCTGGAG | ||
| + | | | ||
| + | | | ||
|- | |- | ||
|green | |green | ||
| Line 28: | Line 36: | ||
|LdhA | |LdhA | ||
|ttgtgctataaacggcgagtttcat | |ttgtgctataaacggcgagtttcat | ||
| + | | | ||
| + | | | ||
|- | |- | ||
|blue | |blue | ||
| Line 33: | Line 43: | ||
|pta | |pta | ||
|ttcacgacaacgttcaataatcat | |ttcacgacaacgttcaataatcat | ||
| + | | | ||
| + | | | ||
|- | |- | ||
|pink | |pink | ||
| Line 38: | Line 50: | ||
|adhE | |adhE | ||
|TTACTAAAAAAGTTTAACATTATCA | |TTACTAAAAAAGTTTAACATTATCA | ||
| + | | | ||
| + | | | ||
|- | |- | ||
|purple | |purple | ||
| Line 43: | Line 57: | ||
|adhE | |adhE | ||
|TTTACTAAAAAAGTTTAACATTATC | |TTTACTAAAAAAGTTTAACATTATC | ||
| + | | | ||
| + | | | ||
|- | |- | ||
|white | |white | ||
| Line 48: | Line 64: | ||
| adhE | | adhE | ||
|CTGATAATGTTAAACTTTTT | |CTGATAATGTTAAACTTTTT | ||
| + | | | ||
| + | | | ||
|} | |} | ||
| Line 57: | Line 75: | ||
|'''Gene targeted by CRISPRi gRNA''' | |'''Gene targeted by CRISPRi gRNA''' | ||
|'''gRNA sequence (without tag at 3' end)''' | |'''gRNA sequence (without tag at 3' end)''' | ||
| + | |'''Locus targeted (eg. beginning of gene, putative promoter, -35 region)''' | ||
| + | |'''Target template or nontemplate strand''' | ||
|- | |- | ||
|red | |red | ||
| Line 62: | Line 82: | ||
| ack (indirectly, pta) | | ack (indirectly, pta) | ||
| GTTTTTTTAGCCACGTATCAATTAT | | GTTTTTTTAGCCACGTATCAATTAT | ||
| + | | | ||
| + | | | ||
|- | |- | ||
|orange | |orange | ||
| Line 67: | Line 89: | ||
|ldhA | |ldhA | ||
|ATTCAACATCACTGGAGAAAGTCTT | |ATTCAACATCACTGGAGAAAGTCTT | ||
| + | | | ||
| + | | | ||
|- | |- | ||
|blue | |blue | ||
| Line 72: | Line 96: | ||
| ackA | | ackA | ||
| TTTTTAGCCACGTATCAATTAT | | TTTTTAGCCACGTATCAATTAT | ||
| + | | | ||
| + | | | ||
|} | |} | ||
Revision as of 01:06, 1 November 2017
Fermentation product and gene targeted:
T/R
| Team | Ethanol (E) or Acetate (A) | Gene targeted by CRISPRi gRNA | gRNA sequence (without tag at 3' end) | Locus targeted (eg. beginning of gene, putative promoter, -35 region) | Target template or nontemplate strand |
| red | Acetate | adhE | TTAACGCACTCGTAGAGCGTGTAAA | ||
| orange | Ethanol | pta-ack | CTATGGCTCCCTGACGTTTT | ||
| yellow | Ethanol | frdA | GCTGTGGGATAAAAACAATCTGGAG | ||
| green | E | LdhA | ttgtgctataaacggcgagtttcat | ||
| blue | E | pta | ttcacgacaacgttcaataatcat | ||
| pink | Acetate | adhE | TTACTAAAAAAGTTTAACATTATCA | ||
| purple | Acetate | adhE | TTTACTAAAAAAGTTTAACATTATC | ||
| white | Acetate | adhE | CTGATAATGTTAAACTTTTT |
W/F
| Team | Ethanol (E) or Acetate (A) | Gene targeted by CRISPRi gRNA | gRNA sequence (without tag at 3' end) | Locus targeted (eg. beginning of gene, putative promoter, -35 region) | Target template or nontemplate strand |
| red | Ethanol | ack (indirectly, pta) | GTTTTTTTAGCCACGTATCAATTAT | ||
| orange | Ethanol | ldhA | ATTCAACATCACTGGAGAAAGTCTT | ||
| blue | Ethanol | ackA | TTTTTAGCCACGTATCAATTAT |
