Difference between revisions of "Talk:20.109(F17):Design gRNA for CRISPRi (Day2)"
From Course Wiki
(→T/R) |
(→T/R) |
||
| Line 20: | Line 20: | ||
|- | |- | ||
|yellow | |yellow | ||
| − | | | + | |Ethanol |
| − | | | + | |frdA |
| − | | | + | |GCTGTGGGATAAAAACAATCTGGAG |
|- | |- | ||
|green | |green | ||
Revision as of 19:44, 17 October 2017
Fermentation product and gene targeted:
T/R
| Team | Ethanol (E) or Acetate (A) | Gene targeted by CRISPRi gRNA | gRNA sequence (without tag at 3' end) |
| red | Acetate | adhE | CTTAACGCACTCGTAGAGCGTGTAAA |
| orange | Ethanol | pta-ack | CTATGGCTCCCTGACGTTTT |
| yellow | Ethanol | frdA | GCTGTGGGATAAAAACAATCTGGAG |
| green | E | IdhA | |
| blue | |||
| pink | Acetate | adhE | TTACTAAAAAAGTTTAACATTATCA |
| purple | |||
| white | Acetate | adhE | CTGATAATGTTAAACTTTTT |
W/F
| Team | Ethanol (E) or Acetate (A) | Gene targeted by CRISPRi gRNA | gRNA sequence (without tag at 3' end) |
| red | |||
| orange | |||
| green | |||
| blue |
