Difference between revisions of "Talk:20.109(F17):Design gRNA for CRISPRi (Day2)"
From Course Wiki
(→T/R) |
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| Line 12: | Line 12: | ||
| Acetate | | Acetate | ||
| adhE | | adhE | ||
| − | | | + | | CTTAACGCACTCGTAGAGCGTGTAAA |
|- | |- | ||
|orange | |orange | ||
Revision as of 19:39, 17 October 2017
Fermentation product and gene targeted:
T/R
| Team | Ethanol (E) or Acetate (A) | Gene targeted by CRISPRi gRNA | gRNA sequence (without tag at 3' end) |
| red | Acetate | adhE | CTTAACGCACTCGTAGAGCGTGTAAA |
| orange | Ethanol | pta-ack | CTATGGCTCCCTGACGTTTT |
| yellow | |||
| green | E | IdhA | |
| blue | |||
| pink | Acetate | adhE | TTACTAAAAAAGTTTAACATTATCA |
| purple | |||
| white | Acetate | adhE | CTGATAATGTTAAACTTTTT |
W/F
| Team | Ethanol (E) or Acetate (A) | Gene targeted by CRISPRi gRNA | gRNA sequence (without tag at 3' end) |
| red | |||
| orange | |||
| green | |||
| blue |
