Difference between revisions of "Talk:20.109(F17):Design gRNA for CRISPRi (Day2)"
From Course Wiki
(→Fermentation product and gene targeted:) |
|||
| (10 intermediate revisions by 9 users not shown) | |||
| Line 10: | Line 10: | ||
|- | |- | ||
|red | |red | ||
| − | | | + | | Ethanol |
| adhE | | adhE | ||
| − | | | + | | CTTAACGCACTCGTAGAGCGTGTAAA |
|- | |- | ||
|orange | |orange | ||
| Line 20: | Line 20: | ||
|- | |- | ||
|yellow | |yellow | ||
| − | | | + | |Ethanol |
| − | | | + | |frdA |
| − | | | + | |GCTGTGGGATAAAAACAATCTGGAG |
|- | |- | ||
|green | |green | ||
|E | |E | ||
| − | | | + | |LdhA |
| − | | | + | |ttgtgctataaacggcgagtttcat |
|- | |- | ||
|blue | |blue | ||
| − | | | + | |E |
| − | | | + | |pta |
| − | | | + | |ttcacgacaacgttcaataatcat |
|- | |- | ||
|pink | |pink | ||
| Line 40: | Line 40: | ||
|- | |- | ||
|purple | |purple | ||
| − | | | + | |Acetate |
| − | | | + | |adhE |
| − | | | + | |GATTTACTAAAAAAGTTTAACATTATC |
|- | |- | ||
|white | |white | ||
| Line 59: | Line 59: | ||
|- | |- | ||
|red | |red | ||
| − | | | + | | Ethanol |
| − | | | + | | ack (indirectly, pta) |
| − | | | + | | GTTTTTTTAGCCACGTATCAATTAT |
|- | |- | ||
|orange | |orange | ||
| − | | | + | |Ethanol |
| − | | | + | |ldhA |
| − | | | + | |ATTCAACATCACTGGAGAAAGTCTT |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
|- | |- | ||
|blue | |blue | ||
| − | | | + | | Ethanol |
| − | | | + | | ackA |
| − | | | + | | TTTTTAGCCACGTATCAATTAT |
|} | |} | ||
Latest revision as of 18:39, 16 October 2018
Fermentation product and gene targeted:
T/R
| Team | Ethanol (E) or Acetate (A) | Gene targeted by CRISPRi gRNA | gRNA sequence (without tag at 3' end) |
| red | Ethanol | adhE | CTTAACGCACTCGTAGAGCGTGTAAA |
| orange | Ethanol | pta-ack | CTATGGCTCCCTGACGTTTT |
| yellow | Ethanol | frdA | GCTGTGGGATAAAAACAATCTGGAG |
| green | E | LdhA | ttgtgctataaacggcgagtttcat |
| blue | E | pta | ttcacgacaacgttcaataatcat |
| pink | Acetate | adhE | TTACTAAAAAAGTTTAACATTATCA |
| purple | Acetate | adhE | GATTTACTAAAAAAGTTTAACATTATC |
| white | Acetate | adhE | CTGATAATGTTAAACTTTTT |
W/F
| Team | Ethanol (E) or Acetate (A) | Gene targeted by CRISPRi gRNA | gRNA sequence (without tag at 3' end) |
| red | Ethanol | ack (indirectly, pta) | GTTTTTTTAGCCACGTATCAATTAT |
| orange | Ethanol | ldhA | ATTCAACATCACTGGAGAAAGTCTT |
| blue | Ethanol | ackA | TTTTTAGCCACGTATCAATTAT |
