Difference between revisions of "Talk:20.109(F17):Design gRNA for CRISPRi (Day2)"
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|ldhA | |ldhA | ||
|ATTCAACATCACTGGAGAAAGTCTT | |ATTCAACATCACTGGAGAAAGTCTT | ||
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|blue | |blue | ||
Revision as of 19:24, 18 October 2017
Fermentation product and gene targeted:
T/R
| Team | Ethanol (E) or Acetate (A) | Gene targeted by CRISPRi gRNA | gRNA sequence (without tag at 3' end) |
| red | Acetate | adhE | CTTAACGCACTCGTAGAGCGTGTAAA |
| orange | Ethanol | pta-ack | CTATGGCTCCCTGACGTTTT |
| yellow | Ethanol | frdA | GCTGTGGGATAAAAACAATCTGGAG |
| green | E | LdhA | ttgtgctataaacggcgagtttcat |
| blue | E | pta | ttcacgacaacgttcaataatcat |
| pink | Acetate | adhE | TTACTAAAAAAGTTTAACATTATCA |
| purple | Acetate | adhE | GATTTACTAAAAAAGTTTAACATTATC |
| white | Acetate | adhE | CTGATAATGTTAAACTTTTT |
W/F
| Team | Ethanol (E) or Acetate (A) | Gene targeted by CRISPRi gRNA | gRNA sequence (without tag at 3' end) |
| red | Ethanol | ack (indirectly, pta) | GTTTTTTTAGCCACGTATCAATTAT |
| orange | Ethanol | ldhA | ATTCAACATCACTGGAGAAAGTCTT |
| blue | Ethanol | ackA | TTTTTAGCCACGTATCAATTAT |
