Talk:20.109(F16):Module 1
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Module 1 Data
M1D2: cell loading parameters
All teams' choices are recorded here.
The images from the doubling time experiments, CometChip t = 0 (see below), are illustrative of how many cells were loaded per microwell under each condition.
M1D2: doubling time, pictures and fluorescence signal
The 'data' below refer to Excel spreadsheets
- listing the fluorescence signal (a.u., with 1 cell corresponding to ~ 1600 a.u.) detected in each microwell in the field of view (rows 8 and above),
- together with the average signal in representative macrowells of quadrants A1, A2, B1, and B2 (row 5; see loading conditions above),
- and with the corresponding calculated doubling times (row 6).
One image per quadrant was recorded. Open images in ImageJ for all encoded information to be displayed.
team | data summary | t=0 and t=2.5d images |
T/R yellow | data | images |
T/R green | data | images |
T/R blue | data | images |
T/R pink | data | images |
T/R purple | data | images |
W/F red | data | images |
W/F green | data | images |
W/F blue | data | images |
W/F purple | data | images |
M1D3: H2O2 and MMS dose response pictures and comet analysis
One image per macrowell was collected. Open images in ImageJ for all encoded information to be displayed.
The 'data' below refer to Excel spreadsheets in which
- rows A, B, C were triplicates in 'live' CometChips,
- rows D, E, F in 'dead' CometChips,
- and columns 1-6 correspond to the 6 drug doses the cells were treated with:
- H2O2: 0, 10, 20, 40, 60, and 80 μM
- MMS: 0, 0.25, 0.5, 1, 2, and 4 mM.
- The 'comet number' tab indicates how many comets were analyzed in each image.
- The other tabs represent various options to quantify DNA damage.
team | data summary | images |
T/R yellow | data | images |
T/R green | data | images |
T/R blue | data | images |
T/R pink | data | images |
T/R purple | data | images |
W/F red | data | images |
W/F green | data | images |
W/F blue | data | images |
W/F purple | data | images |