Difference between revisions of "Talk:20.109(F16):Design gRNA for CRISPRi (Day2)"
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Revision as of 19:56, 19 October 2016
Fermentation product and gene targeted:
T/R
Team | Ethanol (E) or lactate (L) | Gene targeted by CRISPRi gRNA | gRNA sequence (without tag at 3' end) |
yellow | L | pflB | TGTCGAAGTACGCAGTAAAT |
green | L | pflB | ATAAAAAATCCACTTAAGAAGGTA |
blue | L | pflB | TTCATTAAGCTCGGACATGTAACA |
pink | L | pflB | AAATAAAAAATCCACTTAAGAAGGT |
purple | L | pflB | AAATCCACTTAAGAAGGTAGGTGT |
W/F
Team | Ethanol (E) or lactate (L) | Gene targeted by CRISPRi gRNA | gRNA sequence (without tag at 3' end) |
red | E | pta-ack | GCC ACG TAT CAA TTA TAG GTA C |
green | E | ldhA | GTAGCTTAAATGTGATTCAACATC |
blue | E | fdrA | CAAGATCGGCTTGAAAGGTTTGCAC |
purple | E | ldhA | TCGTACTGTTTTGTGCTATAAA |