Difference between revisions of "Talk:20.109(F16):Design gRNA for CRISPRi (Day2)"
From Course Wiki
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Revision as of 19:54, 19 October 2016
Fermentation product and gene targeted:
T/R
| Team | Ethanol (E) or lactate (L) | Gene targeted by CRISPRi gRNA | gRNA sequence (without tag at 3' end) |
| yellow | L | pflB | TGTCGAAGTACGCAGTAAAT |
| green | L | pflB | ATAAAAAATCCACTTAAGAAGGTA |
| blue | L | pflB | TTCATTAAGCTCGGACATGTAACA |
| pink | L | pflB | AAATAAAAAATCCACTTAAGAAGGT |
| purple | L | pflB | TCGTACTGTTTTGTGCTATAAA |
W/F
| Team | Ethanol (E) or lactate (L) | Gene targeted by CRISPRi gRNA | gRNA sequence (without tag at 3' end) |
| red | E | pta-ack | GCC ACG TAT CAA TTA TAG GTA C |
| green | E | ldhA | GTAGCTTAAATGTGATTCAACATC |
| blue | E | fdrA | CAAGATCGGCTTGAAAGGTTTGCAC |
| purple | E | ldhA | GGCGAGTTTCATAAGACTTTC |
