Difference between revisions of "Talk:20.109(F15):Induce protein and evaluate DNA (Day3)"
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Mutant 1 (D57H): 2.04 | Mutant 1 (D57H): 2.04 | ||
Mutant 2 (D57H): 1.08 | Mutant 2 (D57H): 1.08 | ||
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WT: 1.42 | WT: 1.42 | ||
Mutant 1: 1.45 | Mutant 1: 1.45 | ||
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| + | |||
Pink Team (W/F) | Pink Team (W/F) | ||
OD600s: | OD600s: | ||
| + | |||
-IPTG (t=0)_1.5mL WT: 0.8, IPC-D132H 1: 0.72, IPC-D132H 2: 0.68 (in 1mL) | -IPTG (t=0)_1.5mL WT: 0.8, IPC-D132H 1: 0.72, IPC-D132H 2: 0.68 (in 1mL) | ||
+IPTG (t=2.5h)_3mL WT: 2.02, IPC-D132H 1: 1.6, IPC-D132H 2: 1.0 | +IPTG (t=2.5h)_3mL WT: 2.02, IPC-D132H 1: 1.6, IPC-D132H 2: 1.0 | ||
Revision as of 23:24, 22 October 2015
Red Team (W/F) OD600 +IPTG Control (wild type): 2.06 Mutant 1 (D57H): 2.04 Mutant 2 (D57H): 1.08
Pink Team (T/R) OD600 -IPTG WT: 1.79 Mutant 1: 1.66 +IPTG WT: 1.42 Mutant 1: 1.45
Pink Team (W/F) OD600s:
-IPTG (t=0)_1.5mL WT: 0.8, IPC-D132H 1: 0.72, IPC-D132H 2: 0.68 (in 1mL)
+IPTG (t=2.5h)_3mL WT: 2.02, IPC-D132H 1: 1.6, IPC-D132H 2: 1.0
