Difference between revisions of "Talk:20.109(F15):Induce protein and evaluate DNA (Day3)"
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WT: 1.42 | WT: 1.42 | ||
Mutant 1: 1.45 | Mutant 1: 1.45 | ||
+ | |||
+ | Pink Team (W/F) | ||
+ | OD600s: | ||
+ | -IPTG (t=0)_1.5mL WT: 0.8, IPC-D132H 1: 0.72, IPC-D132H 2: 0.68 (in 1mL) | ||
+ | +IPTG (t=2.5h)_3mL WT: 2.02, IPC-D132H 1: 1.6, IPC-D132H 2: 1.0 |
Revision as of 23:23, 22 October 2015
Red Team (W/F) OD600 +IPTG Control (wild type): 2.06 Mutant 1 (D57H): 2.04 Mutant 2 (D57H): 1.08
Pink Team (T/R)
OD600
-IPTG
WT: 1.79
Mutant 1: 1.66
+IPTG
WT: 1.42
Mutant 1: 1.45
Pink Team (W/F) OD600s: -IPTG (t=0)_1.5mL WT: 0.8, IPC-D132H 1: 0.72, IPC-D132H 2: 0.68 (in 1mL) +IPTG (t=2.5h)_3mL WT: 2.02, IPC-D132H 1: 1.6, IPC-D132H 2: 1.0