Difference between revisions of "Talk:20.109(F15):Induce protein and evaluate DNA (Day3)"
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Red Team (W/F) | Red Team (W/F) | ||
OD600 | OD600 | ||
| + | -IPTG | ||
| + | Control (wild type): 0.96 | ||
| + | Mutant 2 (D57H): 0.63 | ||
| + | |||
+IPTG | +IPTG | ||
| − | Control (wild type): | + | Control (wild type): 1.2 |
| − | + | ||
Mutant 2 (D57H): 1.08 | Mutant 2 (D57H): 1.08 | ||
| + | |||
| + | |||
| + | |||
| + | Red Team (T/R) | ||
| + | OD600 | ||
| + | |||
| + | ''-IPTG'']] | ||
| + | WT: 1.13; | ||
| + | Mutant: 1.21; | ||
| + | |||
| + | ''+IPTG'']] | ||
| + | WT: 1.27; | ||
| + | Mutant: 1.33; | ||
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WT: 1.42 | WT: 1.42 | ||
Mutant 1: 1.45 | Mutant 1: 1.45 | ||
| + | |||
| + | |||
Pink Team (W/F) | Pink Team (W/F) | ||
OD600s: | OD600s: | ||
| + | |||
-IPTG (t=0)_1.5mL WT: 0.8, IPC-D132H 1: 0.72, IPC-D132H 2: 0.68 (in 1mL) | -IPTG (t=0)_1.5mL WT: 0.8, IPC-D132H 1: 0.72, IPC-D132H 2: 0.68 (in 1mL) | ||
+IPTG (t=2.5h)_3mL WT: 2.02, IPC-D132H 1: 1.6, IPC-D132H 2: 1.0 | +IPTG (t=2.5h)_3mL WT: 2.02, IPC-D132H 1: 1.6, IPC-D132H 2: 1.0 | ||
| + | |||
| + | Yellow (W/F) | ||
| + | OD values: | ||
| + | with induction | ||
| + | WT: 1.86 | ||
| + | D21A 1: 0.91 | ||
| + | D21A 2: 0.76 | ||
| + | without induction | ||
| + | WT: 0.8 | ||
| + | D21A 1: 0.44 | ||
| + | D21A 2: 0.44 | ||
| + | |||
| + | |||
| + | Green Team (W/F) | ||
| + | OD600: | ||
| + | |||
| + | -IPTG: IPC WT: 1.10; T78P #1: 0.91; T78P #2: 0.79 | ||
| + | |||
| + | +IPTG: IPC WT: 2.62; T78P #1: 1.44; T78P #2: 1.90 | ||
| + | |||
| + | |||
| + | Blue Team (W/F) | ||
| + | OD600: | ||
| + | |||
| + | -IPTG: WT: 0.85; E30K #1: 1.07; E30K #2: 1.23 | ||
| + | |||
| + | +IPTG: WT: 1.56; E30K #1: 1.97; E30K #2: 2.29 | ||
Latest revision as of 00:49, 29 August 2016
Red Team (W/F) OD600 -IPTG Control (wild type): 0.96 Mutant 2 (D57H): 0.63
+IPTG Control (wild type): 1.2 Mutant 2 (D57H): 1.08
Red Team (T/R) OD600
-IPTG]] WT: 1.13; Mutant: 1.21;
+IPTG]] WT: 1.27; Mutant: 1.33;
Pink Team (T/R)
OD600
-IPTG
WT: 1.79
Mutant 1: 1.66
+IPTG
WT: 1.42
Mutant 1: 1.45
Pink Team (W/F) OD600s:
-IPTG (t=0)_1.5mL WT: 0.8, IPC-D132H 1: 0.72, IPC-D132H 2: 0.68 (in 1mL)
+IPTG (t=2.5h)_3mL WT: 2.02, IPC-D132H 1: 1.6, IPC-D132H 2: 1.0
Yellow (W/F) OD values: with induction WT: 1.86 D21A 1: 0.91 D21A 2: 0.76 without induction WT: 0.8 D21A 1: 0.44 D21A 2: 0.44
Green Team (W/F)
OD600:
-IPTG: IPC WT: 1.10; T78P #1: 0.91; T78P #2: 0.79
+IPTG: IPC WT: 2.62; T78P #1: 1.44; T78P #2: 1.90
Blue Team (W/F)
OD600:
-IPTG: WT: 0.85; E30K #1: 1.07; E30K #2: 1.23
+IPTG: WT: 1.56; E30K #1: 1.97; E30K #2: 2.29
