Difference between revisions of "Talk:20.109(F15):Induce protein and evaluate DNA (Day3)"

Revision as of 03:57, 27 October 2015

Red Team (W/F) OD600 -IPTG Control (wild type): 0.96 Mutant 2 (D57H): 0.63

+IPTG Control (wild type): 1.2 Mutant 2 (D57H): 1.08

Red Team (T/R) oD600 -IPTG WT: 1.13 Mutant: 1.21 +IPTG WT: 1.27 Mutant: 1.33

Pink Team (T/R) OD600 -IPTG WT: 1.79 Mutant 1: 1.66 +IPTG WT: 1.42 Mutant 1: 1.45

Pink Team (W/F) OD600s:

-IPTG (t=0)_1.5mL WT: 0.8, IPC-D132H 1: 0.72, IPC-D132H 2: 0.68 (in 1mL)

+IPTG (t=2.5h)_3mL WT: 2.02, IPC-D132H 1: 1.6, IPC-D132H 2: 1.0

Yellow (W/F) OD values: with induction WT: 1.86 D21A 1: 0.91 D21A 2: 0.76 without induction WT: 0.8 D21A 1: 0.44 D21A 2: 0.44

Green Team (W/F) OD600:

-IPTG: IPC WT: 1.10; T78P #1: 0.91; T78P #2: 0.79

+IPTG: IPC WT: 2.62; T78P #1: 1.44; T78P #2: 1.90

Blue Team (W/F) OD600:

-IPTG: WT: 0.85; E30K #1: 1.07; E30K #2: 1.23

+IPTG: WT: 1.56; E30K #1: 1.97; E30K #2: 2.29