Difference between revisions of "Talk:20.109(F15):Induce protein and evaluate DNA (Day3)"

Revision as of 03:11, 23 October 2015

Red Team (W/F) OD600 +IPTG Control (wild type): 2.06 Mutant 1 (D57H): 2.04 Mutant 2 (D57H): 1.08

Pink Team (T/R) OD600 -IPTG WT: 1.79 Mutant 1: 1.66 +IPTG WT: 1.42 Mutant 1: 1.45

Pink Team (W/F) OD600s:

-IPTG (t=0)_1.5mL WT: 0.8, IPC-D132H 1: 0.72, IPC-D132H 2: 0.68 (in 1mL)

+IPTG (t=2.5h)_3mL WT: 2.02, IPC-D132H 1: 1.6, IPC-D132H 2: 1.0

Yellow (W/F) OD values: with induction WT: 1.86 D21A 1: 0.91 D21A 2: 0.76 without induction WT: 0.8 D21A 1: 0.44 D21A 2: 0.44

Green Team (W/F) OD600:

-IPTG: IPC WT: 1.10; T78P #1: 0.91; T78P #2: 0.79

+IPTG: IPC WT: 2.62; T78P #1: 1.44; T78P #2: 1.90