Difference between revisions of "Talk:20.109(F15):Induce protein and evaluate DNA (Day3)"
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Jiapei Chen (Talk | contribs) |
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+IPTG (t=2.5h)_3mL WT: 2.02, IPC-D132H 1: 1.6, IPC-D132H 2: 1.0 | +IPTG (t=2.5h)_3mL WT: 2.02, IPC-D132H 1: 1.6, IPC-D132H 2: 1.0 | ||
| + | |||
| + | Yellow (W/F) | ||
| + | OD values: | ||
| + | with induction | ||
| + | WT: 1.86 | ||
| + | D21A 1: 0.91 | ||
| + | D21A 2: 0.76 | ||
| + | without induction | ||
| + | WT: 0.8 | ||
| + | D21A 1: 0.44 | ||
| + | D21A 2: 0.44 | ||
Revision as of 02:10, 23 October 2015
Red Team (W/F) OD600 +IPTG Control (wild type): 2.06 Mutant 1 (D57H): 2.04 Mutant 2 (D57H): 1.08
Pink Team (T/R) OD600 -IPTG WT: 1.79 Mutant 1: 1.66 +IPTG WT: 1.42 Mutant 1: 1.45
Pink Team (W/F) OD600s:
-IPTG (t=0)_1.5mL WT: 0.8, IPC-D132H 1: 0.72, IPC-D132H 2: 0.68 (in 1mL)
+IPTG (t=2.5h)_3mL WT: 2.02, IPC-D132H 1: 1.6, IPC-D132H 2: 1.0
Yellow (W/F) OD values: with induction WT: 1.86 D21A 1: 0.91 D21A 2: 0.76 without induction WT: 0.8 D21A 1: 0.44 D21A 2: 0.44
