Difference between revisions of "Talk:20.109(F15):Evaluate mutations and site-directed mutagenesis (Day1)"

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(→‎IPC mutants (and primers) chosen by teams)
(→‎IPC mutants (and primers) chosen by teams)
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<font color = #FF0000>'''T/R RED'''</font color>
 
<font color = #FF0000>'''T/R RED'''</font color>
 
*mutation: D132H
 
*mutation: D132H
 +
*forward primer:
 +
*reverse primer:
 +
*restriction enzyme:
 +
<font color = #FF0000>'''W/F RED'''</font color>
 +
*mutation:
 
*forward primer:
 
*forward primer:
 
*reverse primer:
 
*reverse primer:
Line 9: Line 14:
 
*forward primer:
 
*forward primer:
 
*reverse primer:
 
*reverse primer:
−
*restriction enzyme:  
+
*restriction enzyme:
 +
<font color = #FFFF00>'''W/F YELLOW'''</font color>
 +
*mutation: D21A
 +
*forward primer:
 +
*reverse primer:
 +
*restriction enzyme: 
 
<br><font color = #00FF00>'''T/R GREEN'''</font color>
 
<br><font color = #00FF00>'''T/R GREEN'''</font color>
 
*mutation: D57H
 
*mutation: D57H
 +
*forward primer:
 +
*reverse primer:
 +
*restriction enzyme:
 +
<br><font color = #00FF00>'''W/F GREEN'''</font color>
 +
*mutation:
 
*forward primer:
 
*forward primer:
 
*reverse primer:
 
*reverse primer:
Line 17: Line 32:
 
<br><font color = #0000FF>'''T/R BLUE'''</font color>
 
<br><font color = #0000FF>'''T/R BLUE'''</font color>
 
*mutation: D132H
 
*mutation: D132H
 +
*forward primer:
 +
*reverse primer:
 +
*restriction enzyme:
 +
<br><font color = #0000FF>'''W/F BLUE'''</font color>
 +
*mutation:
 
*forward primer:
 
*forward primer:
 
*reverse primer:
 
*reverse primer:
Line 25: Line 45:
 
*reverse primer: 5'TCT GCT TCC CTT ATC ATT TC 3'
 
*reverse primer: 5'TCT GCT TCC CTT ATC ATT TC 3'
 
*restriction enzyme: NaeI (GCC / GGC)
 
*restriction enzyme: NaeI (GCC / GGC)
 +
<br><font color = #FF66CC>'''W/F PINK'''</font color>
 +
*mutation:
 +
*forward primer:
 +
*reverse primer:
 +
*restriction enzyme:

Revision as of 19:15, 9 October 2015

IPC mutants (and primers) chosen by teams

T/R RED

  • mutation: D132H
  • forward primer:
  • reverse primer:
  • restriction enzyme:

W/F RED

  • mutation:
  • forward primer:
  • reverse primer:
  • restriction enzyme:


T/R ORANGE

  • mutation: T78P
  • forward primer:
  • reverse primer:
  • restriction enzyme:

W/F YELLOW

  • mutation: D21A
  • forward primer:
  • reverse primer:
  • restriction enzyme:


T/R GREEN

  • mutation: D57H
  • forward primer:
  • reverse primer:
  • restriction enzyme:


W/F GREEN

  • mutation:
  • forward primer:
  • reverse primer:
  • restriction enzyme:


T/R BLUE

  • mutation: D132H
  • forward primer:
  • reverse primer:
  • restriction enzyme:


W/F BLUE

  • mutation:
  • forward primer:
  • reverse primer:
  • restriction enzyme:


T/R PINK

  • mutation: D132H
  • forward primer: 5' GCC GGC TAT CGA TGG TCA TGG CCA AGT AAA C 3'
  • reverse primer: 5'TCT GCT TCC CTT ATC ATT TC 3'
  • restriction enzyme: NaeI (GCC / GGC)


W/F PINK

  • mutation:
  • forward primer:
  • reverse primer:
  • restriction enzyme:
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