Spring 2012:Vincent Lee Lab 2
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Revision as of 15:10, 8 May 2012 by Vincent Lee (Talk | contribs)
Contents
Objectives
- Building a fluorescence microscope, tuning
- Learning how optical components like those from ThorLabs come together and work.
- Learning about stepper control in a lab setting
- Learning about 3-dimensional deconvolution and looking at the differences in image reconstruction for the actual and theoretical PSF.
Materials
Microscope
- Epi-fluorescence Microscope[1] with parts from Thorlabs
Drivers
- Pololu A4983 Stepper Motor Driver Carrier with Voltage Regulators[2]
- Thorlabs 8 mm Travel Stepper Motor Actuator[3]
Processing
- Matlab
- Matlab Image Acquisition Toolbox[4]
- Matlab Allied Vision Technologies Adaptor[5]
- National Instruments USB Data Acquisition[6]
- ImageJ[7]
- ImageJ plugins: Deconvolution Lab[8] and Diffraction PSF 3D[9]. Manually save in the plugins folder in ImageJ.
Subject
- Submicron beads, small enough to be considered point sources
Microscope
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Safety
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Alignment
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