Difference between revisions of "Spring 2012:Vincent Lee Lab 2"

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(Created page with "== Objectives == * Pass 20.345!")
 
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== Objectives ==
 
== Objectives ==
−
* Pass 20.345!
+
* Building a fluorescence microscope, tuning
 +
* Learning how optical components like those from ThorLabs come together and work.
 +
* Learning about stepper control in a lab setting
 +
* Learning about 3-dimensional deconvolution and looking at the differences in image reconstruction for the actual and theoretical PSF.
 +
 
 +
 
 +
== Materials ==
 +
=== Microscope ===
 +
* Epi-fluorescence Microscope<ref>[[Lab_Manual:Optical_Microscopy]]</ref> with parts from Thorlabs
 +
=== Drivers ===
 +
[[File:A4988Diagram.png|200px|thumb|<b>Figure 1.</b> The Pololu A4983 Microstepper with voltage regulators.]]
 +
* Pololu A4983 Stepper Motor Driver Carrier with Voltage Regulators<ref name="A4983">[http://www.pololu.com/catalog/product/1202 A4983 Stepper Motor Driver Carrier with Voltage Regulators]</ref>
 +
* Thorlabs 8 mm Travel Stepper Motor Actuator<ref>[http://www.thorlabs.com/newgrouppage9.cfm?objectgroup_id=1881 8 mm Travel Stepper Motor Actuator]</ref>
 +
=== Processing ===
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* Matlab
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* Matlab Image Acquisition Toolbox<ref>[http://www.mathworks.com/products/imaq/ Mathworks Image Acquisition Toolbox]</ref>
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* Matlab Allied Vision Technologies Adaptor<ref>[http://www.alliedvisiontec.com/emea/products/software/3rd-party-solutions/mathworks.html Matlab Allied Vision Technologies Adaptor]</ref>
 +
* National Instruments USB Data Acquisition<ref>[http://sine.ni.com/nips/cds/view/p/lang/en/nid/203223 National Instruments USB Data Acquisition 6210]</ref>
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* ImageJ<ref>[http://rsbweb.nih.gov/ij/ ImageJ - Image Processing and Analysis in Java]</ref>
 +
* ImageJ plugins: Deconvolution Lab<ref>[http://bigwww.epfl.ch/algorithms/deconvolutionlab/ ImageJ Plugin - DeconvolutionLab]</ref> and Diffraction PSF 3D<ref>[http://www.optinav.com/Diffraction-PSF-3D.htm ImageJ Plugin - Diffraction PSF 3D]</ref>. Manually save in the plugins folder in ImageJ.
 +
=== Subject ===
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* Submicron beads, small enough to be considered point sources
 +
 
 +
== Microscope ==
 +
>
 +
=== Safety ===
 +
>
 +
=== Alignment ===

Revision as of 15:10, 8 May 2012

Objectives

  • Building a fluorescence microscope, tuning
  • Learning how optical components like those from ThorLabs come together and work.
  • Learning about stepper control in a lab setting
  • Learning about 3-dimensional deconvolution and looking at the differences in image reconstruction for the actual and theoretical PSF.


Materials

Microscope

  • Epi-fluorescence Microscope[1] with parts from Thorlabs

Drivers

Figure 1. The Pololu A4983 Microstepper with voltage regulators.
  • Pololu A4983 Stepper Motor Driver Carrier with Voltage Regulators[2]
  • Thorlabs 8 mm Travel Stepper Motor Actuator[3]

Processing

  • Matlab
  • Matlab Image Acquisition Toolbox[4]
  • Matlab Allied Vision Technologies Adaptor[5]
  • National Instruments USB Data Acquisition[6]
  • ImageJ[7]
  • ImageJ plugins: Deconvolution Lab[8] and Diffraction PSF 3D[9]. Manually save in the plugins folder in ImageJ.

Subject

  • Submicron beads, small enough to be considered point sources

Microscope

>

Safety

>

Alignment


Cite error: <ref> tags exist, but no <references/> tag was found

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