Spring 2012:Vincent Lee Lab 1
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Revision as of 07:03, 13 March 2012 by Vincent Lee (Talk | contribs)
Objectives
- Familiarization with optical trapping theory and practice.
- Preparing and trapping E Coli to measure flagella strength/speed
Calibrations
- Calibrations done with 0.97µm silica beads
Trap Stiffness
- PSD data taken at ~70mW, ~80mW, and ~90mW
- Stokes drag data taken at 80mW and 70mW
Position
- Position calibration done with beads stuck in 10M Aqueous NaCl
E Coli
The E Coli were genetically modified to stick to the cover glass of the microscope slide and to spin their flagella in only one direction.
