Difference between revisions of "Spring 2012:Vincent Lee Lab 1"
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== Objectives == | == Objectives == | ||
* Familiarization with optical trapping theory and practice. | * Familiarization with optical trapping theory and practice. | ||
| − | * Preparing and trapping | + | * Preparing and trapping E Coli to measure flagella strength/speed |
== Calibrations == | == Calibrations == | ||
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* Position calibration done with beads stuck in 10M Aqueous NaCl | * Position calibration done with beads stuck in 10M Aqueous NaCl | ||
| − | == | + | == E Coli == |
| − | + | The E Coli were genetically modified to stick to the cover glass of the microscope slide and to spin their flagella in only one direction. | |
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Revision as of 07:03, 13 March 2012
Objectives
- Familiarization with optical trapping theory and practice.
- Preparing and trapping E Coli to measure flagella strength/speed
Calibrations
- Calibrations done with 0.97µm silica beads
Trap Stiffness
- PSD data taken at ~70mW, ~80mW, and ~90mW
- Stokes drag data taken at 80mW and 70mW
Position
- Position calibration done with beads stuck in 10M Aqueous NaCl
E Coli
The E Coli were genetically modified to stick to the cover glass of the microscope slide and to spin their flagella in only one direction.
