Difference between revisions of "Spring 2012:Vincent Lee Lab 1"
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* Calibrations done with 0.97µm silica beads | * Calibrations done with 0.97µm silica beads | ||
| − | === Trap Stiffness | + | === Trap Stiffness === |
| − | + | ||
| − | + | ||
* PSD data taken at ~70mW, ~80mW, and ~90mW | * PSD data taken at ~70mW, ~80mW, and ~90mW | ||
* Stokes drag data taken at 80mW and 70mW | * Stokes drag data taken at 80mW and 70mW | ||
| + | |||
| + | === Position === | ||
| + | * Position calibration done with beads stuck in 10M Aqueous NaCl | ||
== Calibrations == | == Calibrations == | ||
Revision as of 06:59, 13 March 2012
Contents
Objectives
- Familiarization with optical trapping theory and practice.
- Preparing and trapping e coli to measure flagella strength/speed
Calibrations
- Calibrations done with 0.97µm silica beads
Trap Stiffness
- PSD data taken at ~70mW, ~80mW, and ~90mW
- Stokes drag data taken at 80mW and 70mW
Position
- Position calibration done with beads stuck in 10M Aqueous NaCl
