Spring 2012:Leanna Morinishi Lab 1

Lab 1: Optical Trapping

What I wanted to accomplish

• Complete a button that takes in data from a tethered microbead and adjusts the stage position to center it on the tether
  • Try to get nicer calibration data
  • Function that finds the center
  • Feed in recorded data in a simulation
• Look at the properties of the tether, to calculate the persistence and contour lengths

How I did it

• Typing?

Code

If you measured DNA tethers, provide estimates of the persistence and contour lengths. (This will require a calibration with .97 micron polystyrene microspheres.) If you haven't done much curve fitting or you would like some help understanding the theory, stop by during lab open hours and Prof. Nagle or I will get you pointed in the right direction.