Difference between revisions of "Spring 2012:Leanna Morinishi Lab 1"
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| − | + | === What I wanted to accomplish === | |
| + | * Complete a button that takes in data from a tethered microbead and adjusts the stage position to center it on the tether | ||
| + | ** Try to get nicer calibration data | ||
| + | ** Function that finds the center | ||
| + | ** Feed in recorded data in a simulation | ||
| + | * Look at the properties of the tether, to calculate the persistence and contour lengths | ||
| + | |||
| + | === How I did it === | ||
| + | * | ||
| + | * Typing? | ||
| + | |||
| + | === Code === | ||
| + | |||
| + | |||
If you measured DNA tethers, provide estimates of the persistence and contour lengths. (This will require a calibration with .97 micron polystyrene microspheres.) If you haven't done much curve fitting or you would like some help understanding the theory, stop by during lab open hours and Prof. Nagle or I will get you pointed in the right direction. | If you measured DNA tethers, provide estimates of the persistence and contour lengths. (This will require a calibration with .97 micron polystyrene microspheres.) If you haven't done much curve fitting or you would like some help understanding the theory, stop by during lab open hours and Prof. Nagle or I will get you pointed in the right direction. | ||
Revision as of 03:11, 28 February 2012
Lab 1: Optical Trapping
What I wanted to accomplish
- Complete a button that takes in data from a tethered microbead and adjusts the stage position to center it on the tether
- Try to get nicer calibration data
- Function that finds the center
- Feed in recorded data in a simulation
- Look at the properties of the tether, to calculate the persistence and contour lengths
How I did it
- Typing?
Code
If you measured DNA tethers, provide estimates of the persistence and contour lengths. (This will require a calibration with .97 micron polystyrene microspheres.) If you haven't done much curve fitting or you would like some help understanding the theory, stop by during lab open hours and Prof. Nagle or I will get you pointed in the right direction.
