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• Lab Manual: Limits of Detection
  ==Optical trap procedure== ==Optical trap procedure==
  13 KB (2,117 words) - 17:08, 4 January 2016
• Nonlinear regression
  ...ring the the extension. Regardless of the two force conditions chosen, the procedure provides two, datapoints, (<i>x_i</i>, <i>y_i</i>); <i>
  5 KB (774 words) - 18:07, 13 January 2014
• Locating objects in a fluorescent microscopic image
  ==Procedure==
  3 KB (466 words) - 21:54, 4 January 2013
• Electronics Mini-Lab
  ====Procedure====
  35 KB (5,891 words) - 18:46, 7 April 2017
• Aligning the optical trap
  For this part of the procedure, you will need:
  4 KB (645 words) - 22:09, 3 May 2013
• MATLAB: Estimating resolution from a PSF slide image
  ..., and divide by the magnification. In this part of the lab, you will use a procedure inspired by this simple idea to estimate the resolution of your microscope.
  17 KB (1,769 words) - 14:21, 30 September 2017
• Error analysis
  A ''measurement procedure'' is set of actions that strive to reveal the value of an unknown physical ...was obtained just by chance or because of a shortcoming of the measurement procedure. Conclusions drawn from measured quantities must be considered in the conte
  24 KB (3,757 words) - 14:57, 3 September 2018
• Optical Microscopy: Part 1 Report Outline
  ## Procedure ## Procedure
  2 KB (267 words) - 04:11, 27 July 2015
• Optics Bootcamp
  ...ww.thorlabs.com ThorLabs website] can be very helpful. For example, if the procedure calls for an SPW602 spanner wrench and you have no idea what such a thing m * Repeat the procedure at several image and object distances. Do an equal number with the target p
  28 KB (4,857 words) - 20:09, 31 July 2017
• Estimating second order system parameters from noise power spectra using nonlinear regression
  ...r confidence interval like this: Imagine that you repeated the measurement procedure precisely the same way 100 times. Because of random noise alone, the values
  30 KB (4,815 words) - 21:47, 27 December 2015
• 20.109(S08):Characterize protein expression (Day5)
  ...lyse the bacteria, and run whole bacterial extracts on a protein gel. This procedure is called SDS-PAGE, for sodium docecyl sulfate-polyacrylamide gel electroph ...rocess three samples (the three +IPTG extracts) according to the following procedure. You should either time your spins with another group, or balance your tube
  12 KB (1,946 words) - 16:04, 15 June 2015
• 20.109(S08):Preparing cells for analysis (Day4)
  #Follow the Day 3 procedure (trypsin for monolayer culture, EDTA-citrate for alginate culture) to recov ...ated to 50 &deg;C while you prepare your samples. This is required for the procedure to work optimally.
  16 KB (2,751 words) - 16:04, 15 June 2015
• 20.109(S08):DNA engineering/DNA ligation and bacterial transformation (Day 4)
  ...andled gently, specifically kept cold and not vortexed. The transformation procedure is efficient enough for most lab purposes, with efficiencies as high as 10<
  10 KB (1,704 words) - 14:24, 5 June 2015
• 20.109(S08):DNA engineering/Examine candidate clones (Day 5)
  ...rm what is commonly called a “mini-prep.” This term distinguishes the procedure from a “maxi-” or “large scale-prep” which involves a larger volume
  10 KB (1,634 words) - 14:24, 5 June 2015
• 20.109(S08):DNA engineering/FACS analysis (Day 8)
  ...just for counting and not for separating subpopulations of cells, then the procedure is called "flow cytometry," and this is what you will be doing today.
  5 KB (750 words) - 14:24, 5 June 2015
• 20.109(S08):Guidelines for working in the tissue culture facility
  #Bring only the items you need for a particular procedure into the hood to prevent cluttering your working space. Having a clear wor ...t the center or towards the back. Keep the objects needed for the current procedure within reach; keep the others in the back.
  7 KB (1,095 words) - 14:25, 5 June 2015
• 20.109(S07): Bio-material engineering/Rescreening gold binders
  ...you might want to modify the standard protocol. Otherwise, follow the same procedure as you did for the initial library screen.
  5 KB (887 words) - 15:32, 15 June 2015
• 20.109(S07):Guidelines for working in the tissue culture facility
  #Bring only the items you need for a particular procedure into the hood to prevent cluttering your working space. Having a clear wor ...t the center or towards the back. Keep the objects needed for the current procedure within reach; keep the others in the back.
  6 KB (1,050 words) - 15:32, 15 June 2015
• 20.109(F07):Guidelines for working in the tissue culture facility
  #Bring only the items you need for a particular procedure into the hood to prevent cluttering your working space. Having a clear wor ...t the center or towards the back. Keep the objects needed for the current procedure within reach; keep the others in the back.
  6 KB (1,073 words) - 15:54, 15 June 2015
• 20.109(F07): DNA ligation and bacterial transformation
  ...andled gently, specifically kept cold and not vortexed. The transformation procedure is efficient enough for most lab purposes, with efficiencies as high as 10^
  16 KB (2,748 words) - 15:54, 15 June 2015
• 20.109(F08):DNA engineering/Examine candidate clones (Day 5)
  ...rm what is commonly called a “mini-prep.” This term distinguishes the procedure from a “maxi-” or “large scale-prep” which involves a larger volume
  10 KB (1,634 words) - 19:05, 28 July 2015
• 20.109(F08):DNA engineering/DNA ligation and bacterial transformation (Day 4)
  ...andled gently, specifically kept cold and not vortexed. The transformation procedure is efficient enough for most lab purposes, with efficiencies as high as 10<
  10 KB (1,704 words) - 19:05, 28 July 2015
• 20.109(F08):Guidelines for working in the tissue culture facility
  #Bring only the items you need for a particular procedure into the hood to prevent cluttering your working space. Having a clear wor ...t the center or towards the back. Keep the objects needed for the current procedure within reach; keep the others in the back.
  6 KB (1,075 words) - 19:05, 28 July 2015
• 20.109(F08):DNA engineering/FACS analysis (Day 8)
  ...just for counting and not for separating subpopulations of cells, then the procedure is called "flow cytometry," and this is what you will be doing today.
  5 KB (744 words) - 19:05, 28 July 2015
• 20.109(F08): Mod 1 Day 8 FACS analysis
  ...just for counting and not for separating subpopulations of cells, then the procedure is called "flow cytometry," and this is what you will be doing today.
  4 KB (676 words) - 19:05, 28 July 2015
• 20.109(F08): Mod 1 Day 5 Examine candidate clones
  ...rm what is commonly called a “mini-prep.” This term distinguishes the procedure from a “maxi-” or “large scale-prep” which involves a larger volume
  11 KB (1,770 words) - 19:05, 28 July 2015
• 20.109(F08): Mod 1 Day 4 DNA ligations and bacterial transformations
  ...andled gently, specifically kept cold and not vortexed. The transformation procedure is efficient enough for most lab purposes, with efficiencies as high as 10<
  10 KB (1,617 words) - 19:05, 28 July 2015
• 20.109(S09):Site-directed mutagenesis (Day2)
  ...smid DNA you used in your mutagenesis reaction, assuming that the miniprep procedure results in typical concentrations of 1 &mu;g/&mu;L.
  13 KB (2,155 words) - 19:33, 28 July 2015
• 20.109(S09):Bacterial amplification of DNA (Day3)
  ...smid, you might increase the amount of DNA used during the transformation procedure at the end of lab. In between electrophoresis and transformation, we will h
  13 KB (2,203 words) - 19:33, 28 July 2015
• 20.109(S09):Prepare expression system (Day4)
  ...rm what is commonly called a mini-prep. This term distinguishes the procedure from a maxi- or large scale-prep which involves a larger volume
  21 KB (3,468 words) - 19:33, 28 July 2015
• 20.109(S09):Characterize protein expression (Day6)
  ...lyse the bacteria, and run whole bacterial extracts on a protein gel. This procedure is called SDS-PAGE, for sodium docecyl sulfate-polyacrylamide gel electroph ...rocess three samples (the three +IPTG extracts) according to the following procedure. You should either time your spins with another group, or balance your tube
  14 KB (2,203 words) - 19:33, 28 July 2015
• 20.109(F09): Mod 1 Day 4 DNA ligations and bacterial transformations
  ...andled gently, specifically kept cold and not vortexed. The transformation procedure is efficient enough for most lab purposes, with efficiencies as high as 10<
  10 KB (1,761 words) - 19:38, 28 July 2015
• 20.109(F09): Mod 1 Day 5 Examine candidate clones
  ...rm what is commonly called a “mini-prep.” This term distinguishes the procedure from a “maxi-” or “large scale-prep” which involves a larger volume
  11 KB (1,830 words) - 19:38, 28 July 2015
• 20.109(F09): Mod 1 Day 8 FACS analysis
  ...just for counting and not for separating subpopulations of cells, then the procedure is called "flow cytometry," and this is what you will be doing today.
  5 KB (804 words) - 19:38, 28 July 2015
• 20.109(F09):Guidelines for working in the tissue culture facility
  #Bring only the items you need for a particular procedure into the hood to prevent cluttering your working space. Having a clear wor ...t the center or towards the back. Keep the objects needed for the current procedure within reach; keep the others in the back.
  6 KB (1,084 words) - 19:38, 28 July 2015
• 20.109(F10):Guidelines for working in the tissue culture facility
  #Bring only the items you need for a particular procedure into the hood to prevent cluttering your working space. Having a clear wor ...t the center or towards the back. Keep the objects needed for the current procedure within reach; keep the others in the back.
  6 KB (1,084 words) - 21:00, 28 July 2015
• 20.109(F10): Mod 1 Day 4 DNA ligations and bacterial transformations
  ...andled gently, specifically kept cold and not vortexed. The transformation procedure is efficient enough for most lab purposes, with efficiencies as high as 10<
  11 KB (1,791 words) - 21:00, 28 July 2015
• 20.109(F10): Mod 1 Day 5 Examine candidate clones
  ...rm what is commonly called a “mini-prep.” This term distinguishes the procedure from a “maxi-” or “large scale-prep” which involves a larger volume
  11 KB (1,830 words) - 21:00, 28 July 2015
• 20.109(S11):RNA to DNA by RT-PCR (Day5)
  #Resuspend each sample in 44 &mu;L of RNase-free water by the following procedure: ...deg;C while you prepare your samples. This preparation is required for the procedure to work optimally.
  8 KB (1,243 words) - 21:05, 28 July 2015
• 20.109(F11):Guidelines for working in the tissue culture facility
  #Bring only the items you need for a particular procedure into the hood to prevent cluttering your working space. Having a clear wor ...t the center or towards the back. Keep the objects needed for the current procedure within reach; keep the others in the back.
  10 KB (1,639 words) - 21:08, 28 July 2015
• 20.109(F11): Mod 1 Day 4 DNA ligations and bacterial transformations
  ...andled gently, specifically kept cold and not vortexed. The transformation procedure is efficient enough for most lab purposes, with efficiencies as high as 10< ... of the card if you wish. Your card will be all the information about this procedure that you can use for the [[20.109(F11):DNA engineering lab practical| lab p
  12 KB (1,904 words) - 21:08, 28 July 2015
• 20.109(F11): Mod 1 Day 5 Examine candidate clones & tissue culture
  ...rm what is commonly called a mini-prep. This term distinguishes the procedure from a maxi- or large scale-prep which involves a larger volume
  13 KB (2,328 words) - 21:08, 28 July 2015
• 20.109(F11): Mod 1 Day 7 FACS analysis
  ...just for counting and not for separating subpopulations of cells, then the procedure is called "flow cytometry," and this is what you will be doing today.
  5 KB (840 words) - 21:08, 28 July 2015
• 20.109(S13):Bacterial amplification of DNA (Day3)
  ...smid, you might increase the amount of DNA used during the transformation procedure at the end of lab.
  13 KB (2,181 words) - 13:48, 29 July 2015
• 20.109(S13):Purify protein (Day6)
  ...teria and save the whole cell extracts to later run on a protein gel. This procedure is called SDS-PAGE, for sodium docecyl sulfate-polyacrylamide gel electroph ...rocess three samples (the three +IPTG extracts) according to the following procedure. You should either time your spins with another group, or balance your tube
  13 KB (2,134 words) - 13:48, 29 July 2015
• 20.109(S13): Protein engineering report
  ... in Module 1, assuming a niche audience who is somewhat familiar with each procedure. Use the published scientific literature as a guide, and do not omit ''too'
  5 KB (674 words) - 13:48, 29 July 2015
• 20.109(S13):Guidelines for working in the tissue culture facility
  #Bring only the items you need for a particular procedure into the hood to prevent cluttering your working space. Having a clear wor ...t the center or towards the back. Keep the objects needed for the current procedure within reach; keep the others further back and to the side.'''
  7 KB (1,175 words) - 13:48, 29 July 2015
• 20.109(S14):Guidelines for working in the tissue culture facility
  #Bring only the items you need for a particular procedure into the hood to prevent cluttering your work space. Having a clear work s ...t the center or towards the back. Keep the objects needed for the current procedure within reach; keep the others further back and to the side.'''
  7 KB (1,207 words) - 13:59, 29 July 2015
• 20.109(F13): TA notes for module 1
  * Pre-run entire cloning procedure (Day 1-4) and save materials in case something goes wrong in the student's
  16 KB (2,601 words) - 14:02, 29 July 2015
• 20.109(F14): Mod 1 Day 2 Clean and cut DNA
  The procedure below reflects just one approach you may take to solving this assignment wi
  11 KB (1,780 words) - 14:02, 29 July 2015