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  • DNA Melting Part 2: Lock-in Amplifier and Temperature Control
    ==Experimental procedure== ...e a different statistical procedure, if you like. Be sure to document the procedure you used.
    20 KB (3,227 words) - 20:05, 7 November 2017
  • DNA Melting Report Requirements
    # Procedure: #* Document the procedure used to gather your data.
    6 KB (864 words) - 18:50, 16 April 2017
  • DNA melting: Identifying the unknown sample
    ==Procedure== ...>multcompare</tt> function in MATLAB implements a correction to the t-test procedure to assure that the ''family-wise error rate'' (FWER) is less than the signi
    12 KB (1,931 words) - 18:49, 28 November 2017
  • 20.309:Lab Report Guidelines
    ===Explain the apparatus and procedure=== *Addresses shortcomings of the experimental procedure and problems that arose during experimentation
    10 KB (1,534 words) - 21:33, 13 March 2017
  • 20.309:Safety
    * Dispose of all materials properly. Ask an instructor is you are unsure of procedure.
    5 KB (821 words) - 16:50, 10 September 2015
  • Lab Manual: Atomic Force Microscopy (AFM)
    ... the sample and revisited for later samples. It is set in this calibration procedure by moving the laser spot so that the point of maximum sensitivity is arrive ... on having the correct value for "Scan sensitivity" (from your calibration procedure). The "Scan frequency" (lines per second) sets the speed of the tip across
    56 KB (9,320 words) - 20:42, 13 December 2017
  • ThorLabs OTKB optical trapping kit
    ===Alignment procedure=== ''Note: throughout this procedure, the Relay Lens (3A) will be called the Beam Expander Lens and Relay Lens I
    24 KB (4,010 words) - 15:32, 5 August 2010
  • Procedure: Diffusion in biological gels
    [[Category:Procedure]] {{Template:Safety Warning|message=This procedure uses sensitive biological samples. Wear gloves to avoid contamination.}}
    5 KB (878 words) - 23:26, 5 October 2010
  • Procedure: Particle tracking
    [[Category:Procedure]]
    5 KB (736 words) - 22:05, 4 October 2010
  • Microscopy report outline
    ... make. The measurement sections should include subsections that detail the procedure you used, data you gathered, analysis you did, and results you obtained. Yo
    2 KB (351 words) - 17:04, 11 January 2017
  • Optical Microscopy: Part 4 Report Outline
    <li>Procedure <li>Procedure
    3 KB (482 words) - 19:44, 14 March 2016
  • Procedure: Peptide-DNA Tethering Assay
    [[Category:Procedure]] {{Template:Safety Warning|message=This procedure uses sensitive biological samples. Wear gloves to avoid contamination.}}
    283 B (30 words) - 21:46, 23 February 2011
  • Spring 2011:Optical Trapping Lab
    The procedure depicted in the above graphs is repeated for each measurement, in each of t The procedure depicted above is repeated for several laser intensities and for each stage
    10 KB (1,595 words) - 16:29, 28 February 2012
  • DNA Melting: Simulating DNA Melting - Basics
    The procedure for creating the simulation is:
    13 KB (2,026 words) - 22:46, 15 April 2018
  • Photobleaching Model
    It looks like the general procedure is to point light at it and fry it. ...ll]: frequency-domain measurement using lock-in amplifier. A cool-sounding procedure, well suited to the existing DNA melter, and apparently also beneficial bec
    11 KB (1,755 words) - 23:31, 18 July 2011
  • Optical Microscopy: Part 3 Report Outline
    <li>Procedure <li>Procedure
    2 KB (355 words) - 19:04, 7 March 2016
  • Protocols for cell culture
    ...and gloves. Spray gloves with ethanol. (Repeat as necessary throughout the procedure.)
    18 KB (2,896 words) - 18:17, 25 February 2024
  • Protocols
    ...er with 70% ethanol. Periodically spray gloves with ethanol throughout the procedure.
    4 KB (644 words) - 18:24, 15 August 2013
  • Optical Microscopy Part 1: Brightfield Microscopy
    ...nd 1 μm silica microspheres as described in the magnification measurement procedure (40x objective only).
    23 KB (3,758 words) - 22:45, 12 January 2017
  • Optical Microscopy Part 3: Resolution and Stability
    ... falls on the first minimum of the other source’s image. This suggests a procedure for measuring resolution: image a point source; measure the peak-to-trough ...necessary adjustments to your microscope and repeat this particle-tracking procedure to attain sufficient stability:
    7 KB (1,168 words) - 18:12, 29 September 2016
  • Lab Manual: Limits of Detection
    ==Optical trap procedure== ==Optical trap procedure==
    13 KB (2,117 words) - 17:08, 4 January 2016
  • Nonlinear regression
    ...ring the the extension. Regardless of the two force conditions chosen, the procedure provides two, datapoints, (<i>x<sub>i</sub></i>, <i>y<sub>i</sub></i>); <i>
    5 KB (774 words) - 18:07, 13 January 2014
  • Locating objects in a fluorescent microscopic image
    ==Procedure==
    3 KB (466 words) - 21:54, 4 January 2013
  • Electronics Mini-Lab
    ====Procedure====
    35 KB (5,891 words) - 18:46, 7 April 2017
  • Aligning the optical trap
    For this part of the procedure, you will need:
    4 KB (645 words) - 22:09, 3 May 2013
  • MATLAB: Estimating resolution from a PSF slide image
    ..., and divide by the magnification. In this part of the lab, you will use a procedure inspired by this simple idea to estimate the resolution of your microscope.
    17 KB (1,769 words) - 14:21, 30 September 2017
  • Error analysis
    A ''measurement procedure'' is set of actions that strive to reveal the value of an unknown physical ...was obtained just by chance or because of a shortcoming of the measurement procedure. Conclusions drawn from measured quantities must be considered in the conte
    24 KB (3,757 words) - 14:57, 3 September 2018
  • Optical Microscopy: Part 1 Report Outline
    ## Procedure ## Procedure
    2 KB (267 words) - 04:11, 27 July 2015
  • Optics Bootcamp
    ...ww.thorlabs.com ThorLabs website] can be very helpful. For example, if the procedure calls for an SPW602 spanner wrench and you have no idea what such a thing m * Repeat the procedure at several image and object distances. Do an equal number with the target p
    28 KB (4,857 words) - 20:09, 31 July 2017
  • Estimating second order system parameters from noise power spectra using nonlinear regression
    ...r confidence interval like this: Imagine that you repeated the measurement procedure precisely the same way 100 times. Because of random noise alone, the values
    30 KB (4,815 words) - 21:47, 27 December 2015
  • 20.109(S08):Characterize protein expression (Day5)
    ...lyse the bacteria, and run whole bacterial extracts on a protein gel. This procedure is called SDS-PAGE, for sodium docecyl sulfate-polyacrylamide gel electroph ...rocess three samples (the three +IPTG extracts) according to the following procedure. You should either time your spins with another group, or balance your tube
    12 KB (1,946 words) - 16:04, 15 June 2015
  • 20.109(S08):Preparing cells for analysis (Day4)
    #Follow the Day 3 procedure (trypsin for monolayer culture, EDTA-citrate for alginate culture) to recov ...ated to 50 &deg;C while you prepare your samples. This is required for the procedure to work optimally.
    16 KB (2,751 words) - 16:04, 15 June 2015
  • 20.109(S08):DNA engineering/DNA ligation and bacterial transformation (Day 4)
    ...andled gently, specifically kept cold and not vortexed. The transformation procedure is efficient enough for most lab purposes, with efficiencies as high as 10<
    10 KB (1,704 words) - 14:24, 5 June 2015
  • 20.109(S08):DNA engineering/Examine candidate clones (Day 5)
    ...rm what is commonly called a “mini-prep.” This term distinguishes the procedure from a “maxi-” or “large scale-prep” which involves a larger volume
    10 KB (1,634 words) - 14:24, 5 June 2015
  • 20.109(S08):DNA engineering/FACS analysis (Day 8)
    ...just for counting and not for separating subpopulations of cells, then the procedure is called "flow cytometry," and this is what you will be doing today.
    5 KB (750 words) - 14:24, 5 June 2015
  • 20.109(S08):Guidelines for working in the tissue culture facility
    #Bring only the items you need for a particular procedure into the hood to prevent cluttering your working space. Having a clear wor ...t the center or towards the back. Keep the objects needed for the current procedure within reach; keep the others in the back.
    7 KB (1,095 words) - 14:25, 5 June 2015
  • 20.109(S07): Bio-material engineering/Rescreening gold binders
    ...you might want to modify the standard protocol. Otherwise, follow the same procedure as you did for the initial library screen.
    5 KB (887 words) - 15:32, 15 June 2015
  • 20.109(S07):Guidelines for working in the tissue culture facility
    #Bring only the items you need for a particular procedure into the hood to prevent cluttering your working space. Having a clear wor ...t the center or towards the back. Keep the objects needed for the current procedure within reach; keep the others in the back.
    6 KB (1,050 words) - 15:32, 15 June 2015
  • 20.109(F07):Guidelines for working in the tissue culture facility
    #Bring only the items you need for a particular procedure into the hood to prevent cluttering your working space. Having a clear wor ...t the center or towards the back. Keep the objects needed for the current procedure within reach; keep the others in the back.
    6 KB (1,073 words) - 15:54, 15 June 2015
  • 20.109(F07): DNA ligation and bacterial transformation
    ...andled gently, specifically kept cold and not vortexed. The transformation procedure is efficient enough for most lab purposes, with efficiencies as high as 10^
    16 KB (2,748 words) - 15:54, 15 June 2015
  • 20.109(F08):DNA engineering/Examine candidate clones (Day 5)
    ...rm what is commonly called a “mini-prep.” This term distinguishes the procedure from a “maxi-” or “large scale-prep” which involves a larger volume
    10 KB (1,634 words) - 19:05, 28 July 2015
  • 20.109(F08):DNA engineering/DNA ligation and bacterial transformation (Day 4)
    ...andled gently, specifically kept cold and not vortexed. The transformation procedure is efficient enough for most lab purposes, with efficiencies as high as 10<
    10 KB (1,704 words) - 19:05, 28 July 2015
  • 20.109(F08):Guidelines for working in the tissue culture facility
    #Bring only the items you need for a particular procedure into the hood to prevent cluttering your working space. Having a clear wor ...t the center or towards the back. Keep the objects needed for the current procedure within reach; keep the others in the back.
    6 KB (1,075 words) - 19:05, 28 July 2015
  • 20.109(F08):DNA engineering/FACS analysis (Day 8)
    ...just for counting and not for separating subpopulations of cells, then the procedure is called "flow cytometry," and this is what you will be doing today.
    5 KB (744 words) - 19:05, 28 July 2015
  • 20.109(F08): Mod 1 Day 8 FACS analysis
    ...just for counting and not for separating subpopulations of cells, then the procedure is called "flow cytometry," and this is what you will be doing today.
    4 KB (676 words) - 19:05, 28 July 2015
  • 20.109(F08): Mod 1 Day 5 Examine candidate clones
    ...rm what is commonly called a “mini-prep.” This term distinguishes the procedure from a “maxi-” or “large scale-prep” which involves a larger volume
    11 KB (1,770 words) - 19:05, 28 July 2015
  • 20.109(F08): Mod 1 Day 4 DNA ligations and bacterial transformations
    ...andled gently, specifically kept cold and not vortexed. The transformation procedure is efficient enough for most lab purposes, with efficiencies as high as 10<
    10 KB (1,617 words) - 19:05, 28 July 2015
  • 20.109(S09):Site-directed mutagenesis (Day2)
    ...smid DNA you used in your mutagenesis reaction, assuming that the miniprep procedure results in typical concentrations of 1 &mu;g/&mu;L.
    13 KB (2,155 words) - 19:33, 28 July 2015
  • 20.109(S09):Bacterial amplification of DNA (Day3)
    ...smid, you might increase the amount of DNA used during the transformation procedure at the end of lab. In between electrophoresis and transformation, we will h
    13 KB (2,203 words) - 19:33, 28 July 2015
  • 20.109(S09):Prepare expression system (Day4)
    ...rm what is commonly called a mini-prep. This term distinguishes the procedure from a maxi- or large scale-prep which involves a larger volume
    21 KB (3,468 words) - 19:33, 28 July 2015

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