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• Lab Manual: Limits of Detection
  ==Optical trap procedure== ==Optical trap procedure==
  13 KB (2,117 words) - 17:08, 4 January 2016
• Nonlinear regression
  ...ring the the extension. Regardless of the two force conditions chosen, the procedure provides two, datapoints, (<i>x_i</i>, <i>y_i</i>); <i>
  5 KB (774 words) - 18:07, 13 January 2014
• Locating objects in a fluorescent microscopic image
  ==Procedure==
  3 KB (466 words) - 21:54, 4 January 2013
• Electronics Mini-Lab
  ====Procedure====
  35 KB (5,891 words) - 18:46, 7 April 2017
• Aligning the optical trap
  For this part of the procedure, you will need:
  4 KB (645 words) - 22:09, 3 May 2013
• MATLAB: Estimating resolution from a PSF slide image
  ..., and divide by the magnification. In this part of the lab, you will use a procedure inspired by this simple idea to estimate the resolution of your microscope.
  17 KB (1,769 words) - 14:21, 30 September 2017
• Error analysis
  A ''measurement procedure'' is set of actions that strive to reveal the value of an unknown physical ...was obtained just by chance or because of a shortcoming of the measurement procedure. Conclusions drawn from measured quantities must be considered in the conte
  24 KB (3,757 words) - 14:57, 3 September 2018
• Optical Microscopy: Part 1 Report Outline
  ## Procedure ## Procedure
  2 KB (267 words) - 04:11, 27 July 2015
• Optics Bootcamp
  ...ww.thorlabs.com ThorLabs website] can be very helpful. For example, if the procedure calls for an SPW602 spanner wrench and you have no idea what such a thing m * Repeat the procedure at several image and object distances. Do an equal number with the target p
  28 KB (4,857 words) - 20:09, 31 July 2017
• Estimating second order system parameters from noise power spectra using nonlinear regression
  ...r confidence interval like this: Imagine that you repeated the measurement procedure precisely the same way 100 times. Because of random noise alone, the values
  30 KB (4,815 words) - 21:47, 27 December 2015
• 20.109(S08):Characterize protein expression (Day5)
  ...lyse the bacteria, and run whole bacterial extracts on a protein gel. This procedure is called SDS-PAGE, for sodium docecyl sulfate-polyacrylamide gel electroph ...rocess three samples (the three +IPTG extracts) according to the following procedure. You should either time your spins with another group, or balance your tube
  12 KB (1,946 words) - 16:04, 15 June 2015
• 20.109(S08):Preparing cells for analysis (Day4)
  #Follow the Day 3 procedure (trypsin for monolayer culture, EDTA-citrate for alginate culture) to recov ...ated to 50 &deg;C while you prepare your samples. This is required for the procedure to work optimally.
  16 KB (2,751 words) - 16:04, 15 June 2015
• 20.109(S08):DNA engineering/DNA ligation and bacterial transformation (Day 4)
  ...andled gently, specifically kept cold and not vortexed. The transformation procedure is efficient enough for most lab purposes, with efficiencies as high as 10<
  10 KB (1,704 words) - 14:24, 5 June 2015
• 20.109(S08):DNA engineering/Examine candidate clones (Day 5)
  ...rm what is commonly called a “mini-prep.” This term distinguishes the procedure from a “maxi-” or “large scale-prep” which involves a larger volume
  10 KB (1,634 words) - 14:24, 5 June 2015
• 20.109(S08):DNA engineering/FACS analysis (Day 8)
  ...just for counting and not for separating subpopulations of cells, then the procedure is called "flow cytometry," and this is what you will be doing today.
  5 KB (750 words) - 14:24, 5 June 2015
• 20.109(S08):Guidelines for working in the tissue culture facility
  #Bring only the items you need for a particular procedure into the hood to prevent cluttering your working space. Having a clear wor ...t the center or towards the back. Keep the objects needed for the current procedure within reach; keep the others in the back.
  7 KB (1,095 words) - 14:25, 5 June 2015
• 20.109(S07): Bio-material engineering/Rescreening gold binders
  ...you might want to modify the standard protocol. Otherwise, follow the same procedure as you did for the initial library screen.
  5 KB (887 words) - 15:32, 15 June 2015
• 20.109(S07):Guidelines for working in the tissue culture facility
  #Bring only the items you need for a particular procedure into the hood to prevent cluttering your working space. Having a clear wor ...t the center or towards the back. Keep the objects needed for the current procedure within reach; keep the others in the back.
  6 KB (1,050 words) - 15:32, 15 June 2015
• 20.109(F07):Guidelines for working in the tissue culture facility
  #Bring only the items you need for a particular procedure into the hood to prevent cluttering your working space. Having a clear wor ...t the center or towards the back. Keep the objects needed for the current procedure within reach; keep the others in the back.
  6 KB (1,073 words) - 15:54, 15 June 2015
• 20.109(F07): DNA ligation and bacterial transformation
  ...andled gently, specifically kept cold and not vortexed. The transformation procedure is efficient enough for most lab purposes, with efficiencies as high as 10^
  16 KB (2,748 words) - 15:54, 15 June 2015