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• 20.109(S08):Lab basics
  *Procedures for doing tissue culture work.
  903 B (119 words) - 14:24, 5 June 2015
• 20.109(S08):Student presentations (M2D8)
  ...f your own strengths and weaknesses can often help you improve your future work. After you give your presentation today, write a brief self-evaluation. Spe
  9 KB (1,472 words) - 14:24, 5 June 2015
• 20.109(S08):Lab tour
  Our lab is beautifully equipped. We have a fume hood for work that generates hazardous vapors. We have a back room where dishes can be wa
  22 KB (3,631 words) - 14:24, 5 June 2015
• 20.109(S08):Induce protein expression (Day4)
  As evidenced by Nagais work, wild-type inverse pericam is not toxic to BL21(DE3) cells. Although it is ...our cells with IPTG, you will let the resultant protein factories do their work for 2-3 hours. During this time, you will look at the sequencing results fo
  15 KB (2,475 words) - 14:25, 5 June 2015
• 20.109(S08):Guidelines for oral presentations
  ... elements, including organization, clarity, and proper attribution for the work. *Figure out how to work the lights, slide projector, curtains etc before you begin.
  5 KB (761 words) - 14:25, 5 June 2015
• 20.109(S08):Guidelines for writing a lab report
  ... list the name of your partner on your report since she contributed to the work. ... trends, raw data (like a picture of a gel), or a model that explains your work.
  12 KB (1,912 words) - 14:25, 5 June 2015
• 20.109(S08):Guidelines for working in the tissue culture facility
  ...ut most are common practice and will be good habits for any tissue culture work you do. ...y with 70% ethanol. Start from the back and proceed forward. Swab during work if necessary.
  7 KB (1,095 words) - 14:25, 5 June 2015
• 20.109(S08):Data analysis (Day7)
  ...got last time. Begin by analyzing the wild-type protein as a check on your work (your curve should resemble Nagai's Figure 3L), then move on to your mutant ...SingleKD]], and [[Media:Fit_KDn.m| Fit_KDn]]. Move them to the C:/MATLAB72/work folder on your PC.
  14 KB (2,336 words) - 14:25, 5 June 2015
• 20.109(S07): Yeast transformation
  ...lt projects should be tackled with the idea that they can, should and will work. Attitude alone, however, does not overcome pitfalls and there is much to b ...distinguish these possible explanations but it can tell you that something needs to be re-worked.
  19 KB (3,251 words) - 15:32, 15 June 2015
• 20.109(S07):General lab policy
  ... your partner and to others in the lab if you are not up to speed when the work begins. ... have cut yourself (even if you consider it minor), if something broke and needs cleaning up, or if you are on fire.
  2 KB (380 words) - 15:32, 15 June 2015
• 20.109(S07):Lab tour
  Our lab is beautifully equipped. We have a fume hood for work that generates hazardous vapors. We have a back room where dishes can be wa
  22 KB (3,724 words) - 15:32, 15 June 2015
• 20.109(S07):Assignments
  ...ake a figure using the data you have collected. <font color = red> You can work with your lab partner and friends on these assignments but you will hand in <font color= red><b>Late work will be penalized 1/3 of a letter grade for each day late and will not be a
  2 KB (279 words) - 15:32, 15 June 2015
• 20.109(S07): Start-up expression engineering
  ...gulating protein stability, localization and modifications. Thus, from the work we've done so far this term, you may have the idea that gene expression is ...tocol for designing the reverse primer is just one method of many that can work. For example, taking the reverse complement can be done at any stage, makin
  28 KB (4,311 words) - 15:32, 15 June 2015
• 20.109(S07): Bio-material engineering/Rescreening gold binders
  #* needed resources to complete the work (1 slide) ... vary depending on the particulars of your proposal. You will have time to work on the presentation in lab next time as well as ask questions about the for
  5 KB (887 words) - 15:32, 15 June 2015
• 20.109(S07): Expression engineering report
  You are asked to write a formal lab report detailing your work in this module. Specifics for each section of this report are detailed belo *conclusions you can draw from your work, including any uncertainties
  3 KB (479 words) - 15:32, 15 June 2015
• Bryan Hernandez/20.109/Lab notebook/Module 1/Day 3
  When you have a break from the work described below, be sure to examine the plaques you plated last time. Recor ...assay, what is the titer of each stock solution of phage? Please show your work. If the plaques appeared different, please consider how the phage genomes d
  12 KB (2,079 words) - 15:32, 15 June 2015
• 20.109(S07):Guidelines for editing OpenWetWare
  ==Sign your work==
  3 KB (497 words) - 15:32, 15 June 2015
• 20.109(S07):Guidelines for oral presentations
  ... elements, including organization, clarity, and proper attribution for the work. *Figure out how to work the lights, slide projector, curtains etc before you begin.
  4 KB (732 words) - 15:32, 15 June 2015
• Bryan Hernandez/20.109/Lab notebook/Module 1/Day 5
  ...el should take approximately one hour to run. During that hour, you should work on parts two and three of today's protocol.<br>
  11 KB (1,756 words) - 15:32, 15 June 2015
• 20.109(S07):Guidelines for working in the tissue culture facility
  ...y with 70% ethanol. Start from the back and proceed forward. Swab during work if necessary. ...n the hood and maintain plenty of clear space in the center of the hood to work in.
  6 KB (1,050 words) - 15:32, 15 June 2015
• 20.109(S07):Guidelines for writing a lab report
  ... list the name of your partner on your report since she contributed to the work. ... trends, raw data (like a picture of a gel), or a model that explains your work.
  12 KB (1,849 words) - 15:32, 15 June 2015
• Bryan Hernandez/20.109/Lab notebook/Module 2/Day 2
  ...lls is extremely low (~10^-7M). Calcium pumps and gated channels work hard to establish and keep this steep gradient. Inside the cell, calcium is ...range of such techniques. <b> If you remember only one detail from today's work, let it be the importance of choosing an appropriate tool for the measureme
  19 KB (3,069 words) - 15:48, 15 June 2015
• 20.109(S07): Colony PCR
  ...ng so, you may be switching SAGA-subunits and you will have to rethink the work you have planned so far. And if applicable, it will be worth noting the "ne ...review article] that was written to celebrate and highlight this important work on the structure of the SAGA complex. You and your partner will be randomly
  9 KB (1,466 words) - 15:32, 15 June 2015
• 20.109(S07): Growth of phage materials
  # Choose one of the MDS collection to work with, describing in your lab notebook the deletions that the strain include
  4 KB (600 words) - 15:32, 15 June 2015
• 20.109(S07): cDNA synthesis and microarray
  ...polymerases, this enzyme can only add sequence to an existing chain and so needs a short “primer” to begin synthesis. To perform the cDNA synthesis, you ...NA only” pipet tips and solutions and wiping down your bench. You should work on a fresh piece of benchpaper and remember to wear gloves when working wit
  17 KB (2,874 words) - 15:32, 15 June 2015
• 20.109(S07): Genome engineering essay
  ...ling) can be applied as a design tool for biological engineering. Your lab work with M13 will provide the context for your argument. ...erested in it. To do that, you need to describe the larger context for the work, the ways it’s important, and the specific areas your paper will address.
  7 KB (1,122 words) - 15:32, 15 June 2015
• 20.109(S07): Building phage nanowires
  #* needed resources to complete the work (1 slide) ... vary depending on the particulars of your proposal. You will have time to work on the presentation in lab next time as well as ask questions about the for
  2 KB (319 words) - 15:32, 15 June 2015
• 20.109(S07):Bio-material engineering/Screening library
  Today you will work with a yeast strain bearing either the original pCT-CON plasmid, that expre Some of the work for this experiment has already been done for you. Two nights ago, the yeas
  9 KB (1,473 words) - 15:31, 15 June 2015
• Bryan Hernandez/20.109/Lab notebook/Module 1/Day 6
  ...ty Office will speak with us about biosafety as it relates to cell culture work. </font color> # Familiarize yourself with cell culture work by reading [[20.109(S07):Guidelines for working in the tissue culture facil
  9 KB (1,432 words) - 15:31, 15 June 2015
• 20.109
  ...ts at the bench, ones that will increase the likelihood of success in your work and ensure the health and safety of you and those around you. By the end of
  2 KB (360 words) - 15:31, 15 June 2015
• 20.109:Lab basics
  *Procedures for doing tissue culture work.
  1 KB (139 words) - 15:31, 15 June 2015
• Bryan Hernandez/20.109/Lab notebook/Module 1/Day 4
  ...faculty has set up four overnight LB + kan cultures of bacteria for you to work with. If your reactions were unsuccessful then you have been provided with ... enzymes youll use. The following table may be helpful as you plan your work.
  11 KB (1,751 words) - 15:31, 15 June 2015
• Bryan Hernandez/20.109/Lab notebook/Module 1/Day 2
  ...ly complicated so im going to do it a slightly simpler way that will still work just as good.'''
  17 KB (2,960 words) - 15:31, 15 June 2015
• 20.109(S07):Start-up genome engineering
  ...on of your work, in your lab notebook and on the wiki. By documenting your work according to the exercises done today, you will ...ave the same recognition sites? do they leave the same overhang? will they work in the same buffer? at the same temperature? These are some of the prelimin
  32 KB (5,419 words) - 15:31, 15 June 2015
• 20.109(S07): Western analysis
  ...ple, they built a surrogate T7 that was a better template for experimental work, easier to manipulate and analyze, easier to characterize and understand. ...el should take approximately one hour to run. During that hour, you should work on parts two and three of today's protocol.<br>
  13 KB (2,004 words) - 15:31, 15 June 2015
• 20.109(S07): Measuring calcium in vitro
  ...lls is extremely low (~10^-7M). Calcium pumps and gated channels work hard to establish and keep this steep gradient. Inside the cell, calcium is ...range of such techniques. <b> If you remember only one detail from today's work, let it be the importance of choosing an appropriate tool for the measureme
  20 KB (3,237 words) - 15:45, 15 June 2015
• 20.109(S07): Lipofection
  ...2.5 ul of Lipofectamine 2000 in 50 ul OptiMEM. You and your partner should work together to dilute enough "carrier" for 10 lipofections. Let the dilution s ...out the agent(s) you've chosen, thinking about the concentration(s) you'll work with and how you expect the chemicals to affect the fluorescence. <br>
  9 KB (1,436 words) - 15:31, 15 June 2015
• 20.109(S07): Measuring calcium in vivo
  ... the fluorescent signal. You may also want to turn off the room lights and work with only the task light on.
  7 KB (1,103 words) - 15:31, 15 June 2015
• 20.109(S07): Bio-material engineering/PCR of gold binding candidates
  ...discuss the details of the upcoming presentation and you will have time to work on it with your partner. While these reactions are cycling, you and your partner should work on the research proposal that you will present to the class next time (see
  6 KB (1,087 words) - 15:31, 15 June 2015
• 20.109(S07): Bio-material engineering/Optimizing panning
  ... be used to sketch your experimental set up. It may be possible for you to work on the other parts of today's experiments while the yeast are incubating wi #*needed resources to complete the work
  10 KB (1,637 words) - 15:31, 15 June 2015
• 20.109(F07)
  ...ts at the bench, ones that will increase the likelihood of success in your work and ensure the health and safety of you and those around you. By the end of
  2 KB (372 words) - 15:54, 15 June 2015
• 20.109(F07):Guidelines for editing OpenWetWare
  ==Sign your work==
  3 KB (494 words) - 15:54, 15 June 2015
• 20.109(F07):Lab basics
  *Procedures for doing tissue culture work.
  903 B (119 words) - 15:54, 15 June 2015
• 20.109(F07):Guidelines for working in the tissue culture facility
  ...ut most are common practice and will be good habits for any tissue culture work you do. ...y with 70% ethanol. Start from the back and proceed forward. Swab during work if necessary.
  6 KB (1,073 words) - 15:54, 15 June 2015
• 20.109(F07): Assignments
  ...in the "for next time" section of each lab day. <font color = red> You can work with your lab partner, friends and teaching assistants on these assignments <font color= red><b>Late work will be penalized 1/3 of a letter grade for each day late and will not be a
  3 KB (376 words) - 15:54, 15 June 2015
• 20.109(F07):General lab policy
  ... your partner and to others in the lab if you are not up to speed when the work begins. ... have cut yourself (even if you consider it minor), if something broke and needs cleaning up, or if you are on fire.
  2 KB (375 words) - 15:54, 15 June 2015
• 20.109(F07): siRNA design
  In the previous experimental module, your work has focused on DNA. In this experimental module, RNA gets the spotlight. Wh ... convert an effective inhibitor into a useless one. Conversely, siRNAs can work promiscuously and silence non-target genes, leading to effects on genes tha
  21 KB (3,532 words) - 15:54, 15 June 2015
• 20.109(F07):Guidelines for writing a lab report
  ... list the name of your partner on your report since she contributed to the work. ... trends, raw data (like a picture of a gel), or a model that explains your work.
  12 KB (1,850 words) - 15:54, 15 June 2015
• 20.109(F07):Lab tour
  Our lab is beautifully equipped. We have a fume hood for work that generates hazardous vapors. We have a back room where dishes can be wa
  22 KB (3,670 words) - 15:54, 15 June 2015
• 20.109(F07): DNA ligation and bacterial transformation
  When you have a break from the work described below, be sure to examine the plaques you plated last time. Recor ...ch enzymes you are adding, and how many basepairs of DNA this modification needs. Next, use the plasmid map to help you plan at least two restriction digest
  16 KB (2,748 words) - 15:54, 15 June 2015
• 20.109(F07): M13.1
  ...ple, they built a surrogate T7 that was a better template for experimental work, easier to manipulate and analyze, easier to characterize and understand. N ...so read it carefully and then use some or none of the example for your own work.
  19 KB (3,059 words) - 16:00, 15 June 2015
• 20.109(F07): Western analysis
  ..."electrophoresis," is something you're already familiar with from your DNA work. SDS-PAGE uses charge to separate proteins rather than DNA, but is not iden ...el should take approximately one hour to run. During that hour, you should work on part two of today's protocol.<br>
  11 KB (1,724 words) - 15:54, 15 June 2015
• 20.109(F07): Available enzymes
  ... to find or buy some for you. The ones you specify as part of your own lab work will be automatically purchased so don't fret if you don't see them here.
  549 B (99 words) - 15:54, 15 June 2015
• 20.109(F07): Expression engineering report
  You are asked to write a formal lab report detailing your work in this module. Specifics for each section of this report are detailed belo *conclusions you can draw from your work, including any uncertainties
  3 KB (542 words) - 15:54, 15 June 2015
• 20.109(F07):Guidelines for oral presentations
  ... elements, including organization, clarity, and proper attribution for the work. *Figure out how to work the lights, slide projector, curtains etc before you begin.
  4 KB (733 words) - 15:54, 15 June 2015
• 20.109(F07): Genome engineering assessment
  ...oose a section of the M13.1 scaffold in which you will do your refactoring work (you should choose what you want to refactor based on what seems interestin ... that helps p5 sequester the + strands more effectively, perhaps making p5 work more efficiently by reducing competition. <br>
  15 KB (2,532 words) - 15:54, 15 June 2015
• 20.109(F07): Module 3 oral presentations
  * needed resources to complete the work
  5 KB (796 words) - 15:54, 15 June 2015
• 20.109(F07): Growth of phage materials
  ...ientists who can tolerate a mix of p8 proteins on the phage coat for their work, there are phage-display variations that mix and match fusion and wild-type ... oxide, gold, iridium and indium tin oxide are all in-hand thanks to their work (e.g see reference [http://www.sciencemag.org/cgi/content/abstract/312/5775
  10 KB (1,707 words) - 15:54, 15 June 2015
• 20.109(F07): old announcements
  ...color> there will be no lecture on 11.06.07 to give everyone extra time to work on their oral presentations. ...9(F07):_Agarose_gel_electrophoresis | talk page for that day in lab]. Nice work!!
  2 KB (334 words) - 15:54, 15 June 2015
• 20.109(F07): ECD experimental variations
  ...ments that haven't been done before and for letting everyone know how they work.
  4 KB (569 words) - 15:54, 15 June 2015
• 20.109 (F07): Phage by design
  ...equire a precise definition of life (think back to your Module 1 editorial work). In a top down approach, existing cells would be paired down to their mini You’ll work on two simple math problems for today: First, you’ll add a redesigned M13
  17 KB (2,797 words) - 15:54, 15 June 2015
• 20.109(F07): Luciferase assays and RNA prep
  ...makes it science. There may be other ideas and explanations for how things work but when the preponderance of evidence supports an idea, it becomes the sci Scientific work generates evidence-based, internally consistent, and well-tested explanatio
  11 KB (1,890 words) - 15:55, 15 June 2015
• 20.109(F07): Transmission electron microscopy
  ...esearch proposal and its presentation. Next time you will have a chance to work out more details with your lab partner and then share your idea with anothe
  7 KB (1,172 words) - 15:55, 15 June 2015
• 20.109(F07): Journal article discussion
  ... ug, 2 ug, 1 ug and 0.5 ug of each RNA sample that you prepared. Show your work. Save a copy of your answer since you'll need to know this volume to perfor
  9 KB (1,485 words) - 15:55, 15 June 2015
• 20.109(F07): cDNA synthesis and microarray
  ...polymerases, this enzyme can only add sequence to an existing chain and so needs a short “primer” to begin synthesis. To perform the cDNA synthesis, you ...NA only” pipet tips and solutions and wiping down your bench. You should work on a fresh piece of benchpaper and remember to wear gloves when working wit
  17 KB (2,883 words) - 15:55, 15 June 2015
• 20.109(F07): ECD assembly
  ...rovide complete and accurate information as well as for you to "sign" your work so we can attribute you properly. You should also write a summary paragraph
  13 KB (2,320 words) - 15:55, 15 June 2015
• 20.109(F08)
  ...ts at the bench, ones that will increase the likelihood of success in your work and ensure the health and safety of you and those around you. By the end of
  3 KB (514 words) - 19:05, 28 July 2015
• 20.109(F08): Mod 3 Day 7 Oral presentations
  ... you choose, you might build your project after 109 is over, and then your work can be shown in one or more of the international exhibits that are describe ... The topic you choose, however, can be completely unrelated to any ongoing work in our department.
  12 KB (1,972 words) - 19:05, 28 July 2015
• 20.109(F08):Guidelines for editing OpenWetWare
  ==Sign your work==
  3 KB (494 words) - 19:05, 28 July 2015
• 20.109(F08): Phage by design
  ...equire a precise definition of life (think back to your Module 1 editorial work). In a top down approach, existing cells would be paired down to their mini You’ll work on two simple math problems for today: First, you’ll add a redesigned M13
  17 KB (2,797 words) - 19:05, 28 July 2015
• 20.109(F08): Start-up expression engineering
  ...gulating protein stability, localization and modifications. Thus, from the work we've done so far this term, you may have the idea that gene expression is ...tocol for designing the reverse primer is just one method of many that can work. For example, taking the reverse complement can be done at any stage, makin
  29 KB (4,451 words) - 19:05, 28 July 2015
• 20.109(F08): Yeast transformation
  ...lt projects should be tackled with the idea that they can, should and will work. Attitude alone, however, does not overcome pitfalls and there is much to b ...distinguish these possible explanations but it can tell you that something needs to be re-worked.
  19 KB (3,254 words) - 19:05, 28 July 2015
• 20.109(F08): Phage nanowires
  ...ncorrosive, unmachinable, dinosaur killing metal thats relevant for our work in this module. Iridium, when oxidized to IrO2, has a d-orbital conductance ... in sterile water at least 100 ul of the phage concentration you decide to work with and be sure to add this data to your list of [[20.109(F07): ECD experi
  13 KB (2,275 words) - 19:05, 28 July 2015
• 20.109(F08): Colony PCR
  ...ng so, you may be switching SAGA-subunits and you will have to rethink the work you have planned so far. And if applicable, it will be worth noting the "ne ...review article] that was written to celebrate and highlight this important work on the structure of the SAGA complex. You and your partner will be randomly
  9 KB (1,467 words) - 19:05, 28 July 2015
• 20.109(F08): Screen for phenotypes, isolate RNA
  ... Science 1996] A full 10 years later there is still ongoing discussion and work on how to best annotate the genome. [http://www.ncbi.nlm.nih.gov/entrez/que ... different from DNA in its stability. Consequently it is more difficult to work with RNA in the lab. It is not the techniques themselves that are difficult
  21 KB (3,401 words) - 19:05, 28 July 2015
• 20.109(F08): cDNA synthesis and microarray
  ...polymerases, this enzyme can only add sequence to an existing chain and so needs a short “primer” to begin synthesis. To perform the cDNA synthesis, you ...NA only” pipet tips and solutions and wiping down your bench. You should work on a fresh piece of benchpaper and remember to wear gloves when working wit
  17 KB (2,875 words) - 19:05, 28 July 2015
• 20.109(F08): old announcements
  ... will be held this weekend and next week in case you have questions as you work to write up the Mod2 data. [http://openwetware.org/wiki/Natalie_Kuldell | N ...:TAPcollection Nature03.pdf| here (pdf of Nature 2003 paper describing the work)]] and [[Media:TAPcollection Supp Nat03.doc| here (.doc of Supp Info)]] is
  3 KB (565 words) - 19:05, 28 July 2015
• 20.109(F08): Mod 3 Day 1 Growth of phage materials
  ...ientists who can tolerate a mix of p8 proteins on the phage coat for their work, there are phage-display variations that mix and match fusion and wild-type ... oxide, gold, iridium and indium tin oxide are all in-hand thanks to their work (e.g see reference [http://www.sciencemag.org/cgi/content/abstract/312/5775
  16 KB (2,756 words) - 19:05, 28 July 2015
• 20.109(F08): Western Analysis
  ..."electrophoresis," is something you're already familiar with from your DNA work. SDS-PAGE uses charge to separate proteins rather than DNA, but is not iden ...el should take approximately one hour to run. During that hour, you should work on part two of today's protocol.<br>
  11 KB (1,723 words) - 19:05, 28 July 2015
• 20.109(F08): Probe Western
  ...y Office] will speak with us about biosafety as it relates to cell culture work. </font color> ...d [http://www.ebi.ac.uk/clustalw/ CLUSTAL-W]. It's default settings should work fine for this alignment and you do not have to enter your email address unl
  14 KB (2,285 words) - 19:05, 28 July 2015
• 20.109(F08):DNA engineering/DNA engineering by PCR (Day 1)
  ... establish good habits for documentation of your work. By documenting your work according to the exercises done today, you will ...re there are no spaces between or after the letters or your search won’t work. Change the color of the start codon to blue. Repeat the “Find…” proc
  23 KB (3,892 words) - 19:30, 28 July 2015
• 20.109(F08):DNA engineering/Agarose gel electrophoresis (Day 3)
  Ligations generally work best when there is a 1:4 ratio of backbone to insert. You will ligate your
  10 KB (1,806 words) - 19:05, 28 July 2015
• 20.109(F08):DNA engineering/Examine candidate clones (Day 5)
  *work best in the same buffer and at the same temperature.<br> ...faculty has set up four overnight LB + AMP cultures of bacteria for you to work with. Three of these cultures have colonies from the ligation reactions you
  10 KB (1,634 words) - 19:05, 28 July 2015
• 20.109(F08):Guidelines for working in the tissue culture facility
  ...ut most are common practice and will be good habits for any tissue culture work you do. ...y with 70% ethanol. Start from the back and proceed forward. Swab during work if necessary.
  6 KB (1,075 words) - 19:05, 28 July 2015
• 20.109(F08): Growth of phage materials
  ...ientists who can tolerate a mix of p8 proteins on the phage coat for their work, there are phage-display variations that mix and match fusion and wild-type ... oxide, gold, iridium and indium tin oxide are all in-hand thanks to their work (e.g see reference [http://www.sciencemag.org/cgi/content/abstract/312/5775
  10 KB (1,707 words) - 19:05, 28 July 2015
• 20.109(F08): Transmission electron microscopy
  ...esearch proposal and its presentation. Next time you will have a chance to work out more details with your lab partner and then share your idea with anothe
  7 KB (1,172 words) - 19:05, 28 July 2015
• 20.109(F08): ECD assembly
  ...rovide complete and accurate information as well as for you to "sign" your work so we can attribute you properly. You should also write a summary paragraph
  13 KB (2,320 words) - 19:05, 28 July 2015
• 20.109(F08): Mod 2 Day 4 SDS-PAGE, screen for phenotypes
  ...n is just how commonly '''large complexes of proteins carry out the cell's work.''' Indeed, it's reasonable to think that a careful cataloging of the cell' ..., "electrophoresis," is something we're already familiar with from our DNA work. SDS-PAGE uses charge to separate proteins rather than DNA, but is not iden
  25 KB (4,068 words) - 19:05, 28 July 2015
• 20.109(F08): Module 3 oral presentations
  * needed resources to complete the work
  5 KB (796 words) - 19:05, 28 July 2015
• 20.109(F08): Mod 1 Day 1 DNA engineering using PCR
  ... establish good habits for documentation of your work. By documenting your work according to the exercises done today, you will ...re there are no spaces between or after the letters or your search won’t work. Change the color of the start codon to blue. Repeat the “Find…” proc
  22 KB (3,807 words) - 19:29, 28 July 2015
• 20.109(F08): Mod 1 Day 5 Examine candidate clones
  *work best in the same buffer and at the same temperature.<br> ...faculty has set up four overnight LB + AMP cultures of bacteria for you to work with. Three of these cultures have colonies from the ligation reactions you
  11 KB (1,770 words) - 19:05, 28 July 2015
• 20.109(F08):General lab policy
  ... your partner and to others in the lab if you are not up to speed when the work begins. ... have cut yourself (even if you consider it minor), if something broke and needs cleaning up, or if you are on fire.
  2 KB (376 words) - 19:05, 28 July 2015
• 20.109(F08): Mod 1 Day 3 Agarose gel electrophoresis
  Ligations generally work best when there is a 1:4 ratio of backbone to insert. You will ligate your
  11 KB (1,817 words) - 19:05, 28 July 2015
• 20.109(F08): Mod 1 Day 7 Lipofection
  ...n this draft of your assignment in to the faculty''' but by beginning this work today, you'll have time to get feed back and have questions answered. You w
  10 KB (1,701 words) - 19:05, 28 July 2015
• 20.109(F08): Protein engineering research article
  *conclusions you can draw from your work, including any uncertainties *limitations of your work, e.g. what kinds of experiments/controls/samples would have been great to i
  8 KB (1,254 words) - 19:05, 28 July 2015
• 20.109(F08): Assignments
  ...in the "for next time" section of each lab day. <font color = red> You can work with your lab partner, friends and teaching assistants on these assignments <font color= red><b>Late work will be penalized 1/3 of a letter grade for each day late and will not be a
  3 KB (472 words) - 19:05, 28 July 2015
• 20.109(F08): Mod 3 Day 2 Phage nanowires
  ...ncorrosive, unmachinable, dinosaur killing metal thats relevant for our work in this module. Iridium, when oxidized to IrO2, has a d-orbital conductance ...u felt you did well during your presentation and 2 things you feel you can work on. This will be a 2 point FNT assignment that is assigned here to count as
  13 KB (2,079 words) - 19:05, 28 July 2015
• 20.109(F08): Mod 2 Day 1 Protein engineering with PCR
  ...tocol for designing the reverse primer is just one method of many that can work. For example, taking the reverse complement can be done at any stage, makin ...this experimental module will be a formal research article describing your work. Some general requirements for this report are detailed [[20.109(F08):Guide
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• 20.109(F08): Mod 2 Day 2 Yeast transformation
  ...lt projects should be tackled with the idea that they can, should and will work. Attitude alone, however, does not overcome pitfalls and there is much to b ...distinguish these possible explanations but it can tell you that something needs to be re-worked.
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• 20.109(F08): Mod 2 Day 3 Colony PCR and journal article discussion
  ... you'll study for the rest of the module, and you will have to rethink the work you have planned so far. And if applicable, it will be worth noting the "ne ...MID:_15653319 ] that was written to celebrate and highlight this important work on the structure of the SAGA complex. You and your partner will be randomly
  9 KB (1,612 words) - 19:06, 28 July 2015