Difference between revisions of "Resource list: DNA melting and PCR"
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* Kubista, et. al. [http://www.tataa.com/files/documents/molecularaspectsofmedicine2006.pdf The real-time polymerase chain reaction]. ''Molecular Aspects of Medicine'' 27 (2006) 95–125. | * Kubista, et. al. [http://www.tataa.com/files/documents/molecularaspectsofmedicine2006.pdf The real-time polymerase chain reaction]. ''Molecular Aspects of Medicine'' 27 (2006) 95–125. | ||
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===Quantitative methods=== | ===Quantitative methods=== | ||
* C A Heid, J Stevens, K J Livak, et al. [http://genome.cshlp.org/content/6/10/986.full.pdf+html Real time quantitative PCR.] Genome Res. 1996 6: 986-994. | * C A Heid, J Stevens, K J Livak, et al. [http://genome.cshlp.org/content/6/10/986.full.pdf+html Real time quantitative PCR.] Genome Res. 1996 6: 986-994. | ||
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Revision as of 00:29, 30 September 2010
DNA melting thermodynamics
- Ryan T. Koehler and Nicolas Peyret Thermodynamic properties of DNA sequences: characteristic values for the human genome Bioinformatics 2005 21(16):3333-3339; doi:10.1093/bioinformatics/bti530
Use of fluorescent reportes to measure DNA melting curves
- Ririe, et al. Product Differentiation by Analysis of DNA Melting Curves during the Polymerase Chain Reaction. Analytical Biochemistry 245, 154–160 (1997)
- Michael T. Bjorndal and D. Kuchnir Fygenson. DNA Melting in the Presence of Fluorescent Intercalating Oxazole Yellow Dyes Measured with a Gel-Based Assay. Biopolymers, Vol. 65, 40–44 (2002)
Applications of DNA melting curves
- Worm, et. al. In-Tube DNA Methylation Profiling by Fluorescence Melting Curve Analysis Clinical Chemistry 47:7 1183–1189 (2001)
- Marziliano, et. al. Melting Temperature Assay for a UGT1A Gene Variant in Gilbert Syndrome. Clinical Chemistry. 2000;46:423-425.) (Also see: Ahsen, et. al. Limitations of Genotyping Based on Amplicon Melting Temperature. Clinical Chemistry. 2001;47:1331-1332.
PCR
- Saiki, et. al. Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase. Science 29 January 1988: Vol. 239. no. 4839, pp. 487 - 491 DOI: 10.1126/science.2448875
- Kubista, et. al. The real-time polymerase chain reaction. Molecular Aspects of Medicine 27 (2006) 95–125.
Quantitative methods
- C A Heid, J Stevens, K J Livak, et al. Real time quantitative PCR. Genome Res. 1996 6: 986-994.
