Difference between revisions of "QRT-PCR"
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Kristin Kuhn (Talk | contribs) |
Kristin Kuhn (Talk | contribs) |
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Or to [[QRT-PCR:Get_Started | Get Started?]] | Or to [[QRT-PCR:Get_Started | Get Started?]] | ||
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<div class="center" style="width:auto; margin-left:auto; margin-right:auto;"><big>20.345: QRT-PCR</big></div> | <div class="center" style="width:auto; margin-left:auto; margin-right:auto;"><big>20.345: QRT-PCR</big></div> | ||
== Current State == | == Current State == | ||
| + | [[File:Pcr-check-1.jpg|thumb|left|100px|taken 5/8/11]] | ||
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| + | === Optics === | ||
| + | Auto-fluorescence with the fiber optic is still a problem. Although one option is to keep looking for a fiber with sufficiently low auto-fluorescence levels, it may be more worthwhile to redesign the optics setup so that a fiber optic is no longer needed. | ||
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| + | If this latter route is taken, top-down reading (i.e., reading the fluorescence through the cap of the PCR tube) will likely be the best way to avoid unwanted noise from the illuminator. | ||
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| + | === Electronics === | ||
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=== LabView === | === LabView === | ||
| − | ''Go to [[QRT-PCR:LabView-versions | LabView QRT-PCR version info | + | ''Go to [[QRT-PCR:LabView-versions | LabView QRT-PCR version info]] page'' |
== Going Forward == | == Going Forward == | ||
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== Recent Iterations == | == Recent Iterations == | ||
| − | ''Go to [[Spring 11:QRT-PCR | + | ''Go to [[Spring 11:QRT-PCR|Spring 2011 Progress Details]].'' |
== Resources == | == Resources == | ||
Revision as of 19:36, 17 May 2011
Or to Get Started?
20.345: QRT-PCR
Contents
Current State
Optics
Auto-fluorescence with the fiber optic is still a problem. Although one option is to keep looking for a fiber with sufficiently low auto-fluorescence levels, it may be more worthwhile to redesign the optics setup so that a fiber optic is no longer needed.
If this latter route is taken, top-down reading (i.e., reading the fluorescence through the cap of the PCR tube) will likely be the best way to avoid unwanted noise from the illuminator.
Electronics
LabView
Go to LabView QRT-PCR version info page
Going Forward
Recent Iterations
Go to Spring 2011 Progress Details.
