Difference between revisions of "QRT-PCR"

Revision as of 19:07, 17 May 2011

20.345: QRT-PCR

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-== Project Proposal ==
-GOAL: be able to detect a trace amount of known DNA
+Or to [[QRT-PCR:Get_Started | Get Started?]]
-Tasks:
+[[File:Pcr-setup.jpg|200px|center]]
-* cyclic heating
+<div class="center" style="width:auto; margin-left:auto; margin-right:auto;"><big>20.345: QRT-PCR</big></div>
-** heated lid
+== Current State ==
-** accurate and uniform heating
+=== LabView ===
-** sufficiently rapid cooling
+''Go to [[QRT-PCR:LabView-versions | LabView QRT-PCR version info?]] page''
-** accurate temperature readout
-* fluorescence detection
+== Going Forward ==
-** optics design and setup
-** electronics: amplification of signal, noise reduction
-* coding
+== Recent Iterations ==
-** heating control
-** mathifying the input data (fluoresence, etc) to produce useful information (# amplicons)
-** GUI
-Questions:
+''Go to [[Spring 11:QRT-PCR: Progress Notes|Spring 2011 Progress Details]].''
-* exactly how accurate do we need to be?
-* how fast is fast enough for cooling?
+== Resources ==
-*
+=== Research Notes ===
+* [[QRT-PCR:Heated Lid | Heated Lid]]
+=== Useful External Links ===
+* [http://www.heatersplus.com/wire.htm Resistance wire temperature table ]
+* [http://www.heatersplus.com/nichrome.htm Calculations with resistance wire ]
+* [http://learnlabview.blogspot.com/search/label/0010%20-%20Programming%20LabView%20-%20Arrays Simple Labview Tutorial ]
+* [http://pathmicro.med.sc.edu/pcr/realtime-home.htm QRT-PCR Tutorial]