Difference between revisions of "Protocols for making microscopy samples"
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(Created page with "Category:20.309 Category:Optical Microscopy Lab {{Template:20.309}} =Making PSF slides= *Use PS-Speck ORANGE beads, 170nm *Using a small pipette tip, spread 2-3uL of ...") |
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*Let the slides air dry | *Let the slides air dry | ||
*Drop ~5uL glycerol on the slide where the beads are | *Drop ~5uL glycerol on the slide where the beads are | ||
| − | *Cover with cover slip, | + | *Cover gently with cover slip, allow glycerol to spread to edge of cover slips |
*Seal edges with nail polish | *Seal edges with nail polish | ||
Latest revision as of 23:15, 12 September 2016
Making PSF slides
- Use PS-Speck ORANGE beads, 170nm
- Using a small pipette tip, spread 2-3uL of beads around to the size of a 18x18 cover slip (do not draw a circle with permanent marker, as its drying seems to mess up the bead distribution)
- Let the slides air dry
- Drop ~5uL glycerol on the slide where the beads are
- Cover gently with cover slip, allow glycerol to spread to edge of cover slips
- Seal edges with nail polish
