Difference between revisions of "Optical Microscopy: Part 1 Report Outline"

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(Created page with "# Apparatus: #* Draw a block diagram of your microscope, including all optical elements and relevant distances. It is unnecessary to document the details of the mechanical constr...")
 
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# Apparatus:
 
# Apparatus:
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#* Draw a block diagram of your microscope, including all optical elements and relevant distances. It is unnecessary to document the details of the mechanical construction.
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#* Draw a block diagram of your microscope, including all optical elements and relevant distances. It is not necessary to document the details of the mechanical construction.
 
#* Describe your design calculations and considerations.
 
#* Describe your design calculations and considerations.
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#* Why not put in a nice snapshot of your microscope? (optional, but certainly a cherished memory in the making)
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#* Why not put in a nice snapshot of your &lsquot;scope? (optional, but certainly a cherished memory in the making)
 
# Magnification
 
# Magnification
 
## Procedure
 
## Procedure
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## Data
 
## Data
 
##* Include example images.
 
##* Include example images.
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#* Provide examples of images of beads captures with each of the three objectives (10×, 40× and 100×).
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## Analysis and Results
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# Analysis:
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##* Report the nominal and actual magnifications and fields of view you measured in a table.
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#* Clarify the approach and steps you chose to go from the raw images to the inferred magnification and field of view quantitative results for your brightfield microscope.  Show an example calculation.
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##* Document the method you used to find magnification.
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#* Establish how the uncertainty of your measurements will be reported ("± standard deviation", "± standard error", "± percentage of average") and what equation you followed to quantify this uncertainty.
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##* List the error sources that contributed significantly to systematic error and uncertainty in your measurement. To the degree possible, quantify the type and magnitude of the error.
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# Results:
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## Discussion (optional for magnification measurement)
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#* Describe the transmitted bright-field performance of your microscope by calculating its magnification and field of view, with each of the three objectives (10×, 40× and 100×):
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#*Explain any challenges you faced in the magnification measurement.
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#** Include a table with the following values for the 10×, 40× and 100× objectives:
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# Particle diameter measurement
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#*** (Don't forget to indicate units!)
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## Procedure
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#*** Theoretical resolution
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##* Document the samples you used and how you captured images (camera settings, software used, etc…)
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#*** Actual magnification by multiple measures (Air Force Target, Ronchi Ruling)
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## Data
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#*** Actual field of view in the sample plane (FOV)
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##* Include example images.
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#** Quantify the uncertainty on your measurements (with an appropriate number of significant decimal points!).
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## Analysis and Results
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#** After calibration, measure the size of microspheres (and justify the size of the microspheres chosen for each of the objectives).
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##* Report the average size of the microspheres in each sample and a measure of variation.
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# Discussion:
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##* Describe how you measured the microspheres.
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#* Explain why some samples could not be well imaged with some objective(s), and whether the results differed from your expectations.
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##* List the error sources that contributed significantly to systematic error and uncertainty in your measurement. To the degree possible, quantify the type and magnitude of the error.
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#* Identify the limits of accuracy of your microscope and characterize its source of error: Possibly in table format,
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# Discussion
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#** indicate which sources of error contribute to the compounded uncertainty reported,  
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#* Explain any challenges you faced measuring the size of silica microspheres.
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#** whether each source of noise is systematic or random, user- vs. instrument-based,
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#* How did your measurements differ from the reported values? What factors contributed to the difference?
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#** how each source of noise impacts numerically your results,
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#** how predominant or minor each source of noise is toward reaching your conclusions,
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#** which sources of error can be compensated / improved on, and which are intrinsic to your microscope design. 
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#* Explain how multiple measurements improve (or not!) the accuracy of your calculations. 
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# Conclusion / Further Work:
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#* Suggest possible design choices that would improve microscope performance.
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Revision as of 22:27, 9 February 2014

  1. Apparatus:
    • Draw a block diagram of your microscope, including all optical elements and relevant distances. It is not necessary to document the details of the mechanical construction.
    • Describe your design calculations and considerations.
    • Why not put in a nice snapshot of your &lsquot;scope? (optional, but certainly a cherished memory in the making)
  2. Magnification
    1. Procedure
      • Document the samples you used and how you captured images (camera settings, software used, etc…)
    2. Data
      • Include example images.
    3. Analysis and Results
      • Report the nominal and actual magnifications and fields of view you measured in a table.
      • Document the method you used to find magnification.
      • List the error sources that contributed significantly to systematic error and uncertainty in your measurement. To the degree possible, quantify the type and magnitude of the error.
    4. Discussion (optional for magnification measurement)
    • Explain any challenges you faced in the magnification measurement.
  3. Particle diameter measurement
    1. Procedure
      • Document the samples you used and how you captured images (camera settings, software used, etc…)
    2. Data
      • Include example images.
    3. Analysis and Results
      • Report the average size of the microspheres in each sample and a measure of variation.
      • Describe how you measured the microspheres.
      • List the error sources that contributed significantly to systematic error and uncertainty in your measurement. To the degree possible, quantify the type and magnitude of the error.
  4. Discussion
    • Explain any challenges you faced measuring the size of silica microspheres.
    • How did your measurements differ from the reported values? What factors contributed to the difference?
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