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==Limits of detection lab== | ==Limits of detection lab== | ||
Revision as of 18:03, 4 September 2013
Bioinstrumentation scholars: you are welcome to edit and improve this course wiki. Correct, helpful, exquisitely-expressed changes will increase your class participation grade. Alterations not meeting those critera may have the opposite effect.
If you would like to contribute to this site, please request an account.
20.309 course materials
General course information
<DynamicPageList> category = 20.309 General Information </DynamicPageList>
Reserve a lab station here: Bioinstrumentation Lab reservation system
Optical microscopy lab
Code examples and simulations
- Converting Gaussian fit to Rayleigh resolution
- MATLAB: Estimating resolution from a PSF slide image
- Matlab: Scalebars
- Calculating MSD and Diffusion Coefficients
Background reading
- Geometrical optics and ray tracing
- Physical optics and resolution
- Optical aberrations
- Aperture and field stops
- Optical detectors, noise, and the limit of detection
- Manta G032 camera measurements
- Understanding log plots
DNA melting lab
Lab manuals
- Lab Manual:Measuring DNA Melting Curves
- DNA Melting Part 1: Measuring Temperature and Fluorescence
- DNA Melting Report Requirements for Part 1
- DNA Melting: Model function and parameter estimation by nonlinear regression
- DNA Melting: Simulating DNA Melting - Intermediate Topics
- DNA melting: Identifying the unknown sample
- DNA Melting Part 2: Lock-in Amplifier and Temperature Control
- DNA Melting Report Requirements for Part 2
Code examples and simulations
- DNA Melting: Simulating DNA Melting - Basics
- DNA Melting: Simulating DNA Melting - Intermediate Topics
- DNA Melting: Model function and parameter estimation by nonlinear regression
Reference materials
Background reading
Limits of detection lab
<DynamicPageList> category = Limits of Detection Lab </DynamicPageList>
Useful things
Resource lists
<DynamicPageList> category = Resource list </DynamicPageList>
Lab manuals
<DynamicPageList> category = Lab Manuals </DynamicPageList>
Matlab tutorials
<DynamicPageList> category = Matlab </DynamicPageList>
Projects
- eSTORM: super-resolution microscope for the classroom
