Limits of Detection:Report Requirements
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Revision as of 00:17, 1 December 2011 by Steven Nagle (Talk | contribs)
Guidelines
- Carefully read sections in the Optical Trapping and Atomic Force Microscopy lab manuals, as guided by your TA. It would be a good idead to read as much as possible from the manuals before your lab sessions with the TA.
- Gather data as a group, but complete these deliverables on your own. This includes both your code and your written report.
- Upload your code and report as separate documents on Stellar. (Include your name in the filename as usual.)
- Write no more than one paragraph when comments are requested.
- Include plots of your raw data and any additional plots to support your answers.
Deliverables
Optical trap exercise
After working with your TA to gather data, work through Section 4 of the Optical Trapping lab manual and include the following in your Limits of Detection report.
- Report your results for trap stiffness by each of the three methods at the same power level: equipartition, PSD roll-off, and Stokes drag.
- Comment on which method you believe to be the best and provide support using concepts that we've discussed in class and in lab both recently and throughout the semester.
- Report the minimum detectable force in a 100 Hz bandwidth, at your power level.
- Submit all code used to arrive at your results.
Atomic force microscope
- Report your results for cantilever stiffness
- Report the minimum detectable force in a 100 Hz bandwidth if one were to use the cantilever you measured.
- Comment on how this value compares to typical forces in biological systems (e.g. antibody/antigen binding, DNA hybridization, interdomain forces in proteins, etc.)? Use whatever knowledge you may have, or find one or two examples, but don't spend long doing literature searches.
- Comment on how you could modify the cantilever to detect smaller forces and state what you believe to be the smallest detectable force using a silicon nitride cantilever. Which parameters most affect the minimum detectable force? I.e. for smaller forces, do you need a cantilever that's shorter? thinner? wider? Comment on which parameter variations will have the greatest effect. Do you see any constraints on these parameters?
- Submit all code used to arrive at your results.