Difference between revisions of "Limits of Detection:Report Requirements"
From Course Wiki
Steven Nagle (Talk | contribs) |
|||
Line 2: | Line 2: | ||
[[Category:20.309]] | [[Category:20.309]] | ||
[[Category:AFM]] | [[Category:AFM]] | ||
+ | [[Category:Optical Trapping]] | ||
{{Template:20.309}} | {{Template:20.309}} | ||
Line 9: | Line 10: | ||
*The mini-lab report will be due at midnight on 12/13. | *The mini-lab report will be due at midnight on 12/13. | ||
*The report should be answer-book style, like a problem set. | *The report should be answer-book style, like a problem set. | ||
− | *Your report should be in PDF format, submitted to Stellar prior to the deadline. | + | *Your report should be in PDF format, submitted to Stellar prior to the deadline. Include your last name in the filename. |
− | *'''''Submit all the computer code (.m files) that you used in your analysis as a single zip file to Stellar.''''' | + | *'''''Submit all the computer code (.m files) that you used in your analysis as a single, separate zip file to Stellar.''''' |
'''''You will gather data as a group; however, reports must be individually authored. Everything in your report, including computer code, must be entirely your own work.''''' | '''''You will gather data as a group; however, reports must be individually authored. Everything in your report, including computer code, must be entirely your own work.''''' | ||
Line 20: | Line 21: | ||
#AFM | #AFM | ||
##What is the stiffness of the cantilever you measured? | ##What is the stiffness of the cantilever you measured? | ||
− | ##How does the stiffness compare to the value computed from the cantilever's material properties? | + | ##How does the stiffness compare to the value computed from the cantilever's material properties and from the Sader method? |
##What is the smallest force you could measure using that cantiliver? | ##What is the smallest force you could measure using that cantiliver? | ||
− | ##How | + | ##How could you modify the cantilever design to measure smaller forces? What is the smallest force that could practically be detected using a silicon nitride cantilever? |
##How does the minimum detectable force compare to typical forces in biological systems (e.g. antibody/antigen binding, DNA hybridization, interdomain forces in proteins, etc.)? | ##How does the minimum detectable force compare to typical forces in biological systems (e.g. antibody/antigen binding, DNA hybridization, interdomain forces in proteins, etc.)? |
Revision as of 04:07, 1 December 2011
- Before you go to the lab, read the optical trapping lab manual and the the Atomic Force Microscopy lab manual
- Attend an optical trap lab session during the week of 11/28-12/3.
- Attend an AFM lab session during the week of 12/5-12/9.
- The mini-lab report will be due at midnight on 12/13.
- The report should be answer-book style, like a problem set.
- Your report should be in PDF format, submitted to Stellar prior to the deadline. Include your last name in the filename.
- Submit all the computer code (.m files) that you used in your analysis as a single, separate zip file to Stellar.
You will gather data as a group; however, reports must be individually authored. Everything in your report, including computer code, must be entirely your own work.
- Optical trap calibration
- What is your estimate of the trap stiffness as a function of power as determined by the three different calibration methods (equipartition, PSD roll-off, and Stokes drag)?
- Which method is superior? Explain why.
- What is the minimum detectable force as a function of power and bandwidth?
- AFM
- What is the stiffness of the cantilever you measured?
- How does the stiffness compare to the value computed from the cantilever's material properties and from the Sader method?
- What is the smallest force you could measure using that cantiliver?
- How could you modify the cantilever design to measure smaller forces? What is the smallest force that could practically be detected using a silicon nitride cantilever?
- How does the minimum detectable force compare to typical forces in biological systems (e.g. antibody/antigen binding, DNA hybridization, interdomain forces in proteins, etc.)?