In silico cloning of pdCas9 construct

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CRISPRi as a teaching tool for inquiry-based investigations

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<center>developed by Noreen L. Lyell
Instructor in the Department of Biological Engineering, MIT


Overview

Laboratory exercises

Related modules

  1. Using CRISPRi to increase ethanol yield in E. coli MG1655
  2. Optimizing sgRNA design to further increase ethanol yield (coming soon!)
    • Students analyze datasets collected in previous semesters to design an optimized sgRNA sequence with laboratory exercises that can be completed in remote teaching format
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