Difference between revisions of "Final Project -- Nathan S Lachenmyer"
From Course Wiki
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− | + | {| border = "1" align="center" style="color: black; background-color: #ffffcc;" width="85%" class="wikitable" | |
+ | !Key Factors!!Proposed Technical Targets!!Requirements for Success!!Current Practice!!Associated Technical Barriers | ||
+ | |||
+ | |- align="center" | ||
+ | |style="width: 20%;"|OCLSM Cost | ||
+ | |style="width: 20%;"|< $8,000 | ||
+ | |style="width: 20%;"|< $15,000 | ||
+ | |style="width: 20%;"|> $100,000 commercial; ~$30,000 DIY | ||
+ | |style="width: 20%;"|Cheap replacement for PMT & Galvo, etc. | ||
+ | |- | ||
+ | |style="width: 20%;"|OCLSM Scan Time | ||
+ | |style="width: 20%;"|1 frame/second | ||
+ | |style="width: 20%;"|1 frame / 10 seconds | ||
+ | |style="width: 20%;"|10-50 fps | ||
+ | |style="width: 20%;"|Fast scanning mirrors + ADC time | ||
+ | |} | ||
=Confocal Microscopy= | =Confocal Microscopy= |
Revision as of 16:02, 21 March 2012
Key Factors | Proposed Technical Targets | Requirements for Success | Current Practice | Associated Technical Barriers |
---|---|---|---|---|
OCLSM Cost | < $8,000 | < $15,000 | > $100,000 commercial; ~$30,000 DIY | Cheap replacement for PMT & Galvo, etc. |
OCLSM Scan Time | 1 frame/second | 1 frame / 10 seconds | 10-50 fps | Fast scanning mirrors + ADC time |
Contents
Confocal Microscopy
Literature
- Imaging in Neuroscience and Development: A Laboratory Manual / editors, Rafael Yuste and Arthur Konnerth
- Confocal microscopy for biologists / Alan R. Hibbs.
- Handbook of biological confocal microscopy / editor, James B. Pawley.
Goals and Checkpoints
- 2 Weeks: Brightfield Confocal Microscopy
- 2 Weeks: Software Image Capture, PSF Characterized, Z-Stack Images
- 3 Weeks: Laser Scanning Features
Proposed Design
Challenges
- Control software
- Getting good resolution