DNA Melting Report Requirements for Part 1

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20.309: Biological Instrumentation and Measurement

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Plot fluorescence versus time for one minute and estimate the noise level by calculating the variance. Using the same amplifier gain, estimate the change in signal between DNA sample in and sample out. What is the signal to noise ratio (SNR) in units of dB for your system? Ensure that your system has an SNR of at least 12 dB. Obtain a DNA melting curve by plotting fluorescence versus temperature. Repeat two more times. On the same graph, plot your three melting curves. Pick your favorite of the three melting curves and use your code from the simulation to perform a least squares fit. What values do you obtain for ΔH and ΔS?

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