Difference between revisions of "DNA Melting Report Requirements for Part 1"
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##Repeat two more times. | ##Repeat two more times. | ||
##On one graph, plot your three melting curves. | ##On one graph, plot your three melting curves. | ||
| − | #Pick your favorite of the three melting curves and use your code from the simulation to perform a least squares fit. | + | #Pick your favorite of the three melting curves and use your code from the simulation to perform a least squares fit and comment on the results. |
##What values do you obtain for ΔH and ΔS? | ##What values do you obtain for ΔH and ΔS? | ||
Revision as of 14:47, 10 October 2012
Report requirements
- Plot fluorescence versus time for one minute and estimate the noise level by calculating the variance.
- Using the same amplifier gain, estimate the change in signal between DNA sample in and sample out.
- What is the signal to noise ratio (SNR) in units of dB for your system?
- Ensure that your system has an SNR of at least 12 dB.
- Obtain a DNA melting curve by plotting fluorescence versus temperature.
- Repeat two more times.
- On one graph, plot your three melting curves.
- Pick your favorite of the three melting curves and use your code from the simulation to perform a least squares fit and comment on the results.
- What values do you obtain for ΔH and ΔS?
Lab manual sections
- DNA Melting: Simulating DNA Melting - Basics
- DNA Melting Part 1: Measuring Temperature and Fluorescence
- DNA Melting Report Requirements for Part 1
- DNA Melting: Simulating DNA Melting - Intermediate Topics
- DNA Melting Part 2: Lock-in Amplifier and Temperature Control
- DNA Melting Report Requirements for Part 2
